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Porcine reproductive and respiratory syndrome virus (PRRSV) is an arterial RNA virus first identified in the United States in 1987. PRRSV virions consist of a positive-strand RNA genome and a nucleocapsid protein (N). The genome of PRRSV is an unsegmented single-stranded positive-stranded RNA of approximately 15 kb in length with a 5′ end cap structure and a 3′ end Poly(A) tail. The nucleocapsid protein envelope of PRRSV contains six structural proteins: membrane protein M, small envelope protein E, N-glycosylated glycoproteins GP 2, GP 3, GP 4 and GP 5. Porcine reproductive and respiratory syndrome (PRRS) is a highly contagious infectious disease caused by PRRSV, characterized by reproductive disorders in pregnant sows and respiratory symptoms in piglets, which seriously affects pig populations around the world and causes considerable economic losses to the pig industry.
Figure 1. Lung histopathology during acute PRRSV infection (Whitworth, et al.2016).
Porcine Reproductive and Respiratory Syndrome Virus ELISA Kit Development
Effective surveillance of PRRSV infection requires sample collection in the field and accurate testing in the laboratory. Several serological methods have been developed to detect PRRSV-specific antibodies in pig serum to determine PRRSV infection status in herds and individual pigs, such as: indirect immunofluorescence assay (IFA), immunoperoxidase monolayer assay (IPMA), and enzyme-linked immunosorbent assay (ELISA). IPMA and IFA require replication of the virus and are labor intensive, are not suitable for routine testing on a large scale. In contrast, ELISA is simple, rapid, sensitive and specific, is the gold standard for the diagnosis of porcine PRRSV infection in the pig industry.
BioVenic is a provider of biology reagents & kits, which has been committed to the development of animal virus diagnostic reagents for many years. We have a professional team with extensive experience in protein expression and purification and antibody preparation. We can prepare monoclonal antibodies specific for individual antigens of porcine reproductive and respiratory syndrome virus or antibodies against whole virus multi-antigens, so we can provide you with a range of customized ELISA kits for porcine reproductive and respiratory syndrome virus detection according to your needs. If you have any needs, please feel free to contact us. We will provide you with high-quality products for animal diagnostics.
The ELISA Kits We Can Develop
| PRRSV IgM-ELISA kit | PRRSV IgE-ELISA kit |
| PRRSV IgN-ELISA kit | PRRSV whole-virus ELISA kit |
| PRRSV GP3-ELISA kit | PRRSV GP5-ELISA kit |
Workflow of ELISA Kit Development
| Workflow | Details |
| Submit ELISA kit development requests | Determine the ELISA development protocol and estimate the cost and cycle based on the assay targets and experimental requirements. |
| Antigen preparation | Recombinant E. coli cells bearing GP3(or GP5, etc.)gene were cultured in liquid medium and induced to express the antigen. Then the antigen is purified and the purity and immunogenicity of GP5 protein were examined by SDS-PAGE and Western blot. |
| Antibody preparation | Preparation of monoclonal antibodies specific for individual antigens of PRRSV or antibodies against whole virus multi-antigens. |
| ELISA kit development | ELISA procedure, optimization of ELISA working conditions, validation of ELISA kits. |
| ELISAkit delivery | We will provide you with customized ELISA kits and product quality inspection report. |
Delivery
A series of ELISA kits for detection porcine reproductive and respiratory syndrome virus
Product quality inspection report (COA)
Other experimental data you need
Our Advantages
- Highly sensitive and specific detection of antibodies
- Less equipment and greater flexibility
- Each kit is rigorously validated and tested
- Enables differentiation of weak from strong positive samples
- Reasonable price and short turnaround time
References
- Sattler, Tatjana, et al. "Comparison of different commercial ELISAs for detection of antibodies against porcine respiratory and reproductive syndrome virus in serum." BMC Veterinary Research 10.1 (2014): 1-6.
- Van Breedam, Wander, et al. "Porcine reproductive and respiratory syndrome virus entry into the porcine macrophage." Journal of General Virology 91.7 (2010): 1659-1667.
- Whitworth, Kristin M., et al. "Gene-edited pigs are protected from porcine reproductive and respiratory syndrome virus." Nature Biotechnology 34.1 (2016): 20-22.
