Mouse anti Pig IgA

Description:

Mouse anti pig IgA antibody, clone K61 1B4 recognizes porcine immunoglobulin (Ig) alpha chain. No cross-reactivity is observed with either porcine IgM or IgG.
It has been suggested that pigs possess two subclasses of IgA, referred to as either IgA1 and IgA2 similar to the human IgA subclasses. More recent research indicates that these are allotypic variants and described as IgAa and IgAb. Research undertaken in connection with porcine IgA response to Foot and Mouth disease has demonstrated that clone K61 1B4 recognises both IgAa and IgAb allotypes.

IgA is the dominant immunoglobulin found in surface secretions where its role is in the protection of body surfaces. High levels of IgA may be detected in tracheal secretions, saliva, intestinal fluid and urogenital tract secretions. The primary function of IgA is to prevent adherence of bacteria and virus to epithelial surfaces. IgA may also act within epithelial cells where it has a role in interrupting viral replication.

Product Form:

Purified IgG - liquid.

Preparation:

Purified IgG prepared by affinity chromatography on protein A from tissue culture supernatant.

Buffer :

Phosphate buffered saline.

Preservative Stabilisers:

0.09% Sodium azide (NaN₃).

Carrier Free:

Yes.

Immunogen:

Porcine IgA.

Approx. Protein Concentrations:

IgG concentration 1.0 mg/ml.

Fusion Partners:

Spleen cells from immunized mice were fused with cells of the mouse P3-X63-Ag8.653 myeloma cell line.

Regulatory:

For research purposes only.

Guarantee:

12 months from date of despatch.

Application:

ELISA, flow cytometry, immunohistology - frozen.

Storage:

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Note:

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.