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Mycoplasma synoviae(MS)is a member of Mycoplasma genus of Mycoplasma family. It has no cell wall and a single-layer membrane structure. MS is one of the main pathogenic bacteria that cause mycoplasma disease. The disease mainly infects poultry, especially chickens aged 4 to 6 weeks, and adult chickens can also develop the disease. Mainly through vertical transmission, but also through the respiratory tract and direct contact. The disease has a long duration and is difficult to eradicate once infected in the flock, resulting in low feed utilization, stunted growth and decreased egg production. The disease is easily mixed with other diseases, which increases the mortality rate and causes huge economic losses to the poultry industry.
Figure 1. Brain from a 47-day-old broiler chicken infected with M. synoviae, in which there is infiltration of primarily lymphocytes in and around the blood vessel (Amer, et al.2019).
Mycoplasma synovialis ELISA Kit Development
Serological methods are commonly used to monitor MS infection, including serum plate agglutination (SPA), hemagglutination inhibition (HI) and enzyme-linked immunosorbent assay (ELISA). Each method has its advantages and disadvantages. SPA detects IgM in serum, while HI and ELISA detect IgG. Therefore, SPA can detect seroconversion several days earlier than HI and ELISA. SPA is used for early screening of MS infection and is prone to false positives and has variable sensitivity between batches of assays. Although HI is more specific than SPA, the antigens required for detection are difficult to obtain in the market, which is not suitable for mass sample screening of MS infection. Compared with SPA and HI, ELISA is widely used for monitoring MS infection in poultry because of its advantages of large number of samples tested, high specificity and easy workflow.
BioVenic is a provider of biology reagents & kits, which has been committed to the development of animal pathogen diagnostic reagents for many years. We have a professional team with extensive experience in protein expression and purification and antibody preparation. We can prepare antibodies specific for antigens of MS, so we can provide you with a range of customized ELISA kits for MS detection according to your needs. If you have any needs, please feel free to contact us. We will provide you with high-quality products for diagnosis of avian diseases.
The ELISA Kits We Can Develop
| MS-H ELISA kit | MS53 ELISA kit |
| WVU-1853 ELISA kit | Other MS ELISA kit |
Workflow of ELISA Kit Development
| Workflow | Details |
| Submit ELISA kit development requests | Determine the ELISA development protocol and estimate the cost and cycle based on the assay targets and experimental requirements. |
| Antigen preparation | Preparation of secreted proteins, extraction of secreted antigens, preparation of soluble antigens of MS. |
| Antibody preparation | Preparation of antibodies specific for MS antigens, analysis for antibodies specificity and epitope. |
| ELISA kit development | Optimizing the best concentration of MS antigens and antibody dilution, optimizing the sensitivity and specificity of ELISA kit. |
| ELISAkit delivery | We will provide you with customized ELISA kits and product quality inspection report. |
Delivery
A series of ELISA kits for detection Mycoplasma synoviae
Product quality inspection report (COA)
Other experimental data you need
Our Advantages
- Highly specificity, low background
- Earlier detection of seroconversion and higher analytical sensitivity
- Each kit is rigorously validated and tested
- Professionals provide you with free consultation
- Reasonable price and short turnaround time
References
- Amer, Mohamed M., Hoda M. Mekky, and Hanaa S. Fedawy. "Molecular identification of Mycoplasma synoviae from breeder chicken flock showing arthritis in Egypt." Veterinary World 12.4 (2019): 535.
- Luciano, R. L., et al. "Comparative study of serological tests for Mycoplasma synoviae diagnosis in commercial poultry breeders." Veterinary Medicine International 2011 (2011).
- Noormohammadi, Amir H., et al. "Improved detection of antibodies to Mycoplasma synoviae vaccine MS-H using an autologous recombinant MSPB enzyme-linked immunosorbent assay." Avian Pathology 31.6 (2002): 611-617.
