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Avian respiratory viruses are viruses that enter the respiratory pathway of birds and are mostly single-stranded RNA viruses. The structure of AIV is typical of avian respiratory viruses (Fig. 1). Avian respiratory viruses can cause respiratory infections in birds. Avian respiratory infections are critical in the poultry industry because poorly managed cases can result in high mortality. The etiology of respiratory diseases is complicated, frequently involving multiple pathogens at the same time. A wide range of pathogens, including avian pneumovirus (APV), avian influenza virus (AIV), and Newcastle disease virus, have been linked to respiratory infections in poultry (NDV). These viruses are of major significance because they can cause disease independently, in association with each other, or in association with bacterial agents.
Figure 1. Scheme of the avian influenza virus (Savinon-Flores, et al., 2021).
Avian Respiratory Virus VN Kit Development
Avian respiratory viruses can infect birds' lungs and cause respiratory infections. When the secretions (saliva, feces) of infected birds are exposed to healthy birds, they become infected with the respiratory virus. With the advancement of medical science and biotechnology in recent years, various methods for detecting these various avian respiratory viruses include virus neutralization have been developed. When using fibroblasts as target cells, virus neutralization inhibits viral attachment to the cell or viral reverse transcription and/or integration, in contrast to molecular biological methods (using macrophages as target cells). This technique can be used to find antibodies, detect diseases (with specific immunosera), or their toxins.
BioVenic is a provider of biology reagents and kits that has been dedicated to the development of animal virus diagnostic reagents for a long time. We can provide you with customized avian respiratory virus VN kits based on your needs. Please feel free to contact us, if you have any questions. We will provide you with high-quality animal diagnostic products and services.
Avian Respiratory Virus VN Kits We Can Provide
| Newcastle disease virus VN kit | Infectious bronchitis virus VN kit | Infectious laryngotracheitis virus VN kit |
| Pulmonary virus VN kit | Influenza A. virus VN kit | Other VN kits you need |
Workflow of Avian Respiratory Virus VN Kit Development
| Workflow | Details |
| Source of virus | Characterized avian influenza virus (AIV) and Newcastle disease (ND) live virus strains were obtained. |
| Source of antibody | Blood samples were collected from commercial breeder farms. |
| Optimization of VNT (variable nozzle turbine) | Variables were optimized during the study for the best neutralization of homologous antigens and antibodies under controlled conditions. |
| Inoculation | Antigen and antisera suspension was centrifuged at 3600g for five minutes. 0.1ml of supernatant was inoculated through the air sac opening. |
| Control group | Serum samples were obtained from non-exposed AIV, and ND vaccinates. The samples were evaluated for relevant virus exposure using homologous antigens. |
| Assessment of the assay | Each of the factors optimized in the study was run five times and data was evaluated through mean standard deviation (M±SD) to get a p-value based on Bonferroni analysis. |
Delivery
- A series of VN kits for avian respiratory viruses
- Product quality inspection report
- Other experimental data you need
Our Advantages
- High sensitivity and high specificity
- High repeatability between tests
- Simple and rapid workflow
- The growth of viral replication can be observed
References
- Proença-Módena, José Luiz, Izolete Santos Macedo, and Eurico Arruda. "H5N1 avian influenza virus: an overview." Brazilian Journal of Infectious Diseases 11 (2007): 125-133.
- Anwar, Huma, et al., "Development and optimization of virus neutralization test in chicken embryonated eggs for indirect identification of avian influenza and Newcastle disease virus." Journal of Drug Delivery and Therapeutics 8.6 (2018): 142-148.
- Savinon-Flores, Fernanda, et al., "A review on SERS-based detection of human virus infections: influenza and coronavirus." Biosensors 11.3 (2021): 66.
