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Influenza virus is a single-stranded RNA virus belonging to the Orthomyxoviridae family, with three types: A, B, and C. Because birds are their natural hosts, influenza A viruses are known as avian influenza viruses (AIV). Avian influenza viruses are type A orthomyxoviruses characterized by homologous nucleoprotein and matrix proteins. AIV's genome is made up of eight singles stranded RNAs packed into separate rod-shaped ribonucleoprotein complexes (RNPs). A viral RNA, a viral polymerase, and several copies of the viral nucleoprotein are all found in each RNP (NP). AI viruses are further divided into 16 hemagglutinin (H1-16) and 9 neuraminidase (N1-9) subtypes. Avian influenza (AIV) is a highly contagious disease that can infect a wide range of poultry and wild birds. It has a great impact on public health and poultry industry and caused massive economic loss.
Figure 1. TEM image of influenza virus (Nuwarda et al., 2021).
Influenza Virus PCR Kit Development
Based on their virulence, AIVs are further classified into low pathogenic avian influenza (LPAI) and high pathogenic avian influenza (HPAI) viruses. Low pathogenicity avian influenza viruses typically produce respiratory signs such as sneezing, coughing, ocular discharge, nasal discharge, and swollen infraorbital sinuses in poultry. High pathogenic avian influenza (HPAI) viruses cause severe, systemic disease with high mortality in chickens and turkeys. Preventive measures are the recommended strategies to prevent birds from AIVs. Molecular diagnostic methods are used as the first step in the identification and control of the IAV outbreaks. Different PCR methods have been implemented in most reference diagnostics laboratories for the detection and characterization of IAVs and among them RT-qPCR is most widely used technique due to its high specificity and high sensitivity.
BioVenic is a provider of biology reagents and kits, which has been committed to the development of animal virus diagnostic reagents for many years. We have a professional team with extensive experience in veterinary diagnostic and reagent development, and our R&D team is working hard to develop and improve PCR kits. We can provide you with a series of customized PCR kits for the detection of influenza virus according to your needs. If you have any needs, please feel free to contact us. We will provide you with high-quality products and services.
Influenza Virus PCR Kit We Can Develop
| Influenza virus TaqMan RT-PCR kit | Influenza virus conventional RT-PCR kit | Influenza virus reverse transcriptase PCR kit |
| Influenza virus multiplex RT-qPCR kit | Influenza virus singleplex real-time RT-PCR kit | Influenza virus RT-PCR kit |
Workflow of Influenza Virus PCR Kit Development
| Workflow | Details |
| Collection of samples | Collection of AIV positive samples from different sources. |
| Primer and probe designing | Professional softwares can be used for the deisgning of primers and probes. |
| Sensitivity analysis | Evaluation of reaction efficiency & detection limit of PCR. |
| PCR condition | Maintenance of specific thermocyclic conditions. |
| Specificity analysis | Evalutaion of specificity of PCR by comparing the AIV-positive samples from different sources. |
Delivery
- A series of PCR kits for influenza virus
- Product quality inspection report
- Other experimental data you need
Our Advantages
- High sensitivity and specificity
- High repeatability between tests
- Each kit is rigorously validated and tested
- Reasonable price
- Simple and rapid workflow
References
- Lee, Dong-Hun, et al. "Evolution, global spread, and pathogenicity of highly pathogenic avian influenza H5Nx clade 2.3. 4.4." Journal of Veterinary Science 18.S1 (2017): 269-280.
- Lee, Chang-Won, et al. "H5N2 avian influenza outbreak in Texas in 2004: the first highly pathogenic strain in the United States in 20 years?." Journal of Virology 79.17 (2005): 11412-11421.
- Nuwarda, Rina Fajri, Abdulsalam Abdullah Alharbi, and Veysel Kayser. "An overview of influenza viruses and vaccines." Vaccines 9.9 (2021): 1032.
