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Mycoplasma gallisepticum (MG) is a cell-wall-less prokaryotic organism belonging to the order Mycoplasma, the family Mycoplasma and the genus Mycoplasma. Chronic respiratory disease (CRD) in chickens is an infectious disease caused by MG that is a serious threat to the chicken industry. Chickens infected with MG alone show respiratory symptoms, but do not cause death in chickens. In recent years, as the scale of chicken breeding increases, feeding methods change and feeding density increases, the incidence of CRD is increasing and its harmfulness is becoming more and more prominent, which mainly affects the growth of chicks and reduces the egg production of adult chickens, causing significant economic losses to the chicken breeding industry.
Figure 1. The morphology of a field strain of Mycoplasma colonies on the Frey's Mycoplasma agar (left, 10X magnifications) and
the Gram's stain Mycoplasma organism (right, 40X magnifications) (Wanasawaeng, et al.2015).
Mycoplasma gallisepticum ELISA Kit Development
Currently, a variety of serological methods have been developed for the detection of MG, including enzyme-linked immunosorbent assay (ELISA), hemagglutination test (HI) and serum panel agglutination (SPA). SPA can detect antibodies from chickens infected with MG at an early stage and is a rapid screening method for MG. It is more sensitive than ELISA and HI. However, SPA is prone to false positive results and sensitivity and specificity may vary from batch to batch. ELISA is a rapid, serologically available method for screening large numbers of sera for MG and is widely used for detection of MG infection in chickens for better control of CRD transmission.
BioVenic is a provider of biology reagents & kits, which has been committed to the development of animal pathogen diagnostic reagents for many years. We have a professional team with extensive experience in protein expression and purification and antibody preparation. We can prepare antibodies specific for antigens of MG, so we can provide you with a range of customized ELISA kits for MG detection according to your needs. If you have any needs, please feel free to contact us. We will provide you with high-quality products for diagnosis of avian diseases.
The ELISA Kits We Can Develop
| MG direct ELISA kit | MG indirect ELISA kit |
| MG DOT- ELISA kit | Other MG ELISA kit |
Workflow of ELISA Kit Development
| Workflow | Details |
| Submit ELISA kit development requests | Determine the ELISA development protocol and estimate the cost and cycle based on the assay targets and experimental requirements. |
| Antigen preparation | Preparation of secreted proteins, extraction of secreted antigens, preparation of soluble antigens of MG. |
| Antibody preparation | Preparation of antibodies specific for MG antigens, analysis for antibodies specificity and epitope. |
| ELISA kit development | Optimizing the best concentration of MG antigens and antibody dilution, optimizing the sensitivity and specificity of ELISA kit. |
| ELISAkit delivery | We will provide you with customized ELISA kits and product quality inspection report. |
Delivery
A series of ELISA kits for detection Mycoplasma gallisepticum
Product quality inspection report (COA)
Other experimental data you need
Our Advantages
- Highly sensitive and specific
- Each kit is rigorously validated and tested
- Allows high sample throughput
- No cross-reactions with other Mycoplasmas
- Reasonable price and short turnaround time
References
- Wanasawaeng, Wisanu, Supawadee Chaichote, and Niwat Chansiripornchai. "Development of ELISA and serum plate agglutination for detecting antibodies of Mycoplasma gallisepticum using strain of Thai isolate." The Thai Journal of Veterinary Medicine 45.4 (2015): 499.
- Kim, Sungwon, et al. "Development and validation of a house finch interleukin-1β (HfIL-1β) ELISA system." BMC Veterinary Research 13.1 (2017): 1-8.
- Yadav, Jay Prakash, et al. "Comparative evaluation of indirect-ELISA and DOT blot assay for serodetection of Mycoplasma gallisepticum and Mycoplasma synoviae antibodies in poultry." Journal of Microbiological Methods 189 (2021): 106317.
