Services
Porcine gastrointestinal pathogens infect farmed pigs from birth through to their marketing age and significantly limit the productivity and profitability of pig production around the world. The main gastrointestinal diseases of pigs are bacterial e.g., colibacillosis due to enterotoxigenic Escherichia coli (ETEC), salmonellosis, swine dysentery due to Brachyspira hyodysenteriae and proliferative enteropathy due to Lawsonia intracellularis infections. The bacterial strains ETEC, Salmonella and Lawsonia all appear to be "embedded" in many pig farms, causing endemic porcine gastrointestinal disease problems. Pigs across Asia also suffer viral enteritis due to coronaviruses, such as porcine epidemic diarrhea virus. Piglets globally suffer the host-specific coccidial parasite, Isospora suis. Parasitic nematode diseases of the pig intestine remain common in some groups of pigs raised outdoors.
Figure 1. Schematic diagram of culture, passaging and usage of enteroids (Luo et al., 2020).
Porcine Gastrointestinal Pathogens AGID Kit Development
As biosecurity management strategies are developed and implemented to prevent introduction and spread of gastrointestinal diseases in pig populations. Detecting the pathogens that cause porcine gastrointestinal diseases in time is crucial. The agar gel immunodiffusion assay, a diagnostic test that employs serum to detect antibodies produced in response to infection, has been used to diagnose porcine gastrointestinal diseases caused by a variety of pathogens. The advantage of the agar gel immunodiffusion test is that it can reliably analyze severely hemolyzed and cytotoxic serum samples. Antibodies are now detected using AGID technologies like radial immunodiffusion assay (RID), double immunodiffusion assay, etc.
For a long time, BioVenic has been developing diagnostic reagents and kits for animal diseases. Our research and development team is efficiently developing and upgrading agar gel immunodiffusion kits, and we have a strong staff with considerable veterinary diagnostic and reagent development experience. Based on your needs, we can provide a variety of agar gel immunodiffusion kits for the detection of porcine gastrointestinal pathogens. If you have any questions, please do not hesitate to contact us. We will provide you with high-quality products and services.
Porcine Gastrointestinal Pathogens AGID Kits We Can Develop
| Escherichia coli radial immunodiffusion enzyme assay kit | Escherichia coli double immunodiffusion assay kit | Brachyspira hyodysenteriae radial immunodiffusion enzyme assay kit |
| Brachyspira hyodysenteriae double immunodiffusion assay kit | Isospora suis double immunodiffusion assay kit | Isospora suis passive immunodiffusion assay kit |
| Lawsonia intracellularis double immunodiffusion assay kit | Lawsonia intracellularis radial immunodiffusion enzyme assay kit | Other AGID kits you need |
Workflow of Porcine Gastrointestinal Pathogens AGID Kit Development
| Workflow | Details |
| Preparation of gel | Agarose gel was prepared and heated for a given period of time. |
| Preparation of reference antigen | Reference antigens were taken from the pigs infected with pathogens. |
| Preparation of antiserum | Antiserum was prepared by collecting the serum from pigs and serum was examined rigorously for specificity. |
| Examination of sample | Samples were diluted using distilled water and conducted for examination. |
| Pathogenic antigen separation | Antigens of pathogens were separated using the centrifugation. |
Delivery
- A set of AGID kits for porcine gastrointestinal pathogens
- Report on product quality
- List of other experimental data you need
Our Advantages
- Short turnaround time
- Detects a small number of swine gastrointestinal infections in a high-sensitivity manner
- Detecting therapeutic pathogens with a higher level of specificity
- Combine AGID with other detection methods to avoid false positives
References
- Luo, Hao, et al. "Utility evaluation of porcine enteroids as PDCoV infection model in vitro." Frontiers in Microbiology 11 (2020): 821.
- V J, Rejish Kumar, et al. "Putative probiotic Lactobacillus spp. from porcine gastrointestinal tract inhibit transmissible gastroenteritis coronavirus and enteric bacterial pathogens." Tropical Animal Health and Production 42.8 (2010): 1855-1860.
- Konstantinov, Sergey R., et al. "Microbial diversity studies of the porcine gastrointestinal ecosystem during weaning transition." Animal Research 53.4 (2004): 317-324.
