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Senecavirus A (SVA), also known as Seneca Valley virus (SVV). It belongs to the genus Senecavirus of the family Picornaviridae. The virions of SVA have an icosahedral structure with a diameter of 25-30 nm. The genome of SVA is a single-stranded positive-stranded RNA with a total length of about 7.3 kb, consisting of a 5'-end non-coding region (5' UTR), an open reading frame (ORF) encoding a polyprotein, and a 3'-end non-coding region (3 'UTR). SVA is an important pathogen that causes blisters on the mouth and hoofs of adult pigs and a large number of deaths in newborn piglets, causing huge economic losses to the pig industry.
Figure 1. Characteristics of Senecavirus A. Transmission electron microscopy of purified SVA virion (a). Schematic representations of SVA virion: front (b) and section (c) views (Liu, Fuxiao, et al. 2020).
Senecavirus A PCR Kit Development
SVA is associated with vesicular disease in weaned and adult pigs and high mortality in newborn piglets. Therefore, developing an effective diagnostic method is crucial to limit the spread of SVA and reduce economic losses. Various methods have been developed to detect SVA, including virus isolation, virus neutralization assay, indirect immunofluorescence assay (IFA), and enzyme-linked immunosorbent assay (ELISA). However, these traditional detection methods often have many defects, such as time-consuming, cumbersome detection process, requiring professional equipment and technicians. In recent years, with the rapid development of biotechnology, various efficient PCR technologies have been developed and used for SVA detection, including conventional RT-PCR, real-time RT-PCR, rRT-PCR, nested PCR, etc. A variety of optimized PCR techniques are increasingly being used to detect SVA because they are not only highly sensitive and efficient, but also avoid the high risk that traditional PCR assays may be hampered by contamination of previously amplified material.
BioVenic is a provider of biology reagents and kits, which has been committed to the development of animal virus diagnostic reagents for many years. We have a professional team with extensive experience in veterinary diagnostic and reagent development, and our R&D team is working hard to develop and improve PCR kits. We can provide you with a series of customized PCR kits for the detection of Senecavirus A according to your needs. If you have any needs, please feel free to contact us. We will provide you with high-quality products and services.
Senecavirus A PCR Kit We Can Develop
| Senecavirus A RT-PCR kit | Senecavirus A real-time RT-PCR kit | Senecavirus A rRT-PCR kit |
| Senecavirus A duplex rRT-PCR kit | Senecavirus A nested PCR kit | Senecavirus A RT-ddPCR kit |
| Senecavirus A qRT-PCR kit | Senecavirus A RT-iiPC kit | Other Senecavirus A PCR kits |
Workflow of Senecavirus A PCR Kit Development
| Workflow | Details |
| RNA extraction | Purify the virus and extract RNA from Senecavirus A -infected cell cultures. |
| Primer design | A set of primers can be designed according to the sequences of specific strains of Senecavirus A using professional software. |
| Establishment of PCR conditions | Maintenance of thermocycling conditions to conduct amplification and detection through PCR. |
| Reproducibility evaluation of the PCR assay | The reproducibility of the PCR kit was assessed by determining the intra-assay and inter-assay coefficients of variation (CV). |
| Assessment of sensitivity and specificity | The specificity of PCR kit was assessed by detection of RNA from Senecavirus A. The sensitivity of the PCR kit was evaluated by determining the limit of detection (LoD). |
Delivery
- A series of PCR kits for Senecavirus A
- Product quality inspection report
- Other experimental data you need
Our Advantages
- High sensitivity and specificity
- High reproducibility between tests
- Minimal cross-contamination
- Reasonable price and short turnaround time
References
- Liu, Fuxiao, et al. "A 5-year review of Senecavirus A in China since its emergence in 2015." Frontiers in Veterinary Science (2020): 694.
- Zhang, Jianqiang, et al. "Development and evaluation of a real-time RT-PCR and a field-deployable RT-insulated isothermal PCR for the detection of Seneca Valley virus." BMC Veterinary Research 15.1 (2019): 1-11.
- Bracht, Alexa J., et al. "Real-time reverse transcription PCR assay for detection of Senecavirus A in swine vesicular diagnostic specimens." PLoS One 11.1 (2016): e0146211.
