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Bovine viral diarrhea virus (BVDV), a member of the genus Pestivirus within the family Flaviviridae, is a single-stranded positive-stranded RNA virus with a capsid. The genome of BVDV consists of a positive-stranded RNA molecule of approximately 12.3 kb in length, including a single open reading frame (ORF) and two are untranslated regions (UTR). Bovine viral diarrhea (BVD) is an infectious disease caused by BVDV, which is widespread in most cattle-raising countries in the world. BVD can be transmitted through contact with the excreta of infected animals, and its high morbidity and mortality can reduce the reproductive performance of cattle and cause continuous economic losses to the cattle industry.
Figure 1. Graphical representation of the genome organization of BVDV. The BVDV genome is composed of a single strand of RNA (the bottom panel). The viral genome encodes a polyprotein (top panel). The central panel showing the composition of the viral genome (the structural and non-structural proteins) (Al-Kubati, et al. 2021).
Bovine Viral Diarrhea Virus ELISA Kit Development
BVD is infectious and can persist and spread in cattle. BVDV detection and culling of infected cattle is an effective measure to control the spread of BVD. Bovine infection with BVDV will produce specific antibodies in serum and cause a strong humoral immune response. Since serum is easy to collect, store and handle, and the survival rate of BVDV in serum is high. Therefore, serological methods are commonly used for BVDV detection, including virus neutralization assay (VNT), enzyme-linked immunosorbent assay (ELISA), and agarose gel immunodiffusion (AGID). Compared to fast and inexpensive ELISA, VNT is relatively expensive and labor-intensive, while AGID is less sensitive to samples with low antibody titers and prone to false-positive results. ELISA is widely used for BVDV detection due to its high specificity and easy standardization.
BioVenic is a provider of biology reagents & kits, which has been committed to the development of animal virus diagnostic reagents for many years. We have a professional team with extensive experience in protein expression and purification and antibody preparation. We can prepare specific antibodies against the different antigens of bovine viral diarrhea virus and can provide you with a series of customized ELISA kits for the detection of bovine viral diarrhea virus according to your needs. If you have any needs, please feel free to contact us. We will provide you with high-quality products for animal diagnostics.
The ELISA Kits We Can Develop
| BVDV p80 Ab ELISA kit | BVDV PI X2 ELISA kit |
| BVDV Total Ab ELISA kit | BVDV E2 ELISA kit |
Workflow of ELISA Kit Development
| Workflow | Details |
|---|---|
| Submit ELISA kit development requests | Determine the ELISA development protocol and estimate the cost and cycle based on the assay targets and experimental requirements. |
| Antigen preparation | DNA extraction, cloning and sequencing of genes encoding the antigens of BVDV, and then constructing vectors to express antigens. |
| Antibody preparation | Preparation of antibodies specific for antigens on BVDV surface. |
| ELISA kit development | Antibody label, antibody paired screening, method development and optimization, kit production. |
| ELISA kit delivery | We will provide you with customized ELISA kits and complete product quality inspection report. |
Delivery
- A series of ELISA kits for detection bovine viral diarrhea virus
- Product quality inspection report (COA)
- Other experimental data you need
Our Advantages
- High sensitivity and specificity
- High repeatability between tests
- Good adsorption performance, low blank value
- Enables detection of all three genotypes of BVDV
- Reasonable price and short turnaround time
References
- Evans, Caitlin A., Sasha R. Lanyon, and Michael P. Reichel. "Investigation of AGID and two commercial ELISAs for the detection of Bovine viral diarrhea virus–specific antibodies in sheep serum." Journal of Veterinary Diagnostic Investigation 29.2 (2017): 181-185.
- Al-Kubati, Anwar AG, et al. "Recent advances on the bovine viral diarrhea virus molecular pathogenesis, immune response, and vaccines development." Frontiers in Veterinary Science 8 (2021): 475.
