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Trichomonas foetus (T. foetus) is a protozoan parasite with a fusiform or pear-shaped body that can infect a variety of animals such as cattle and cats and cause trichomoniasis. The host environment of T. foetus is different in different hosts. It parasitizes the reproductive and gastrointestinal tracts of cattle and cats, respectively. Bovine trichomoniasis is a sexually disease that affects the reproduction of infected cattle and causes severe economic losses to the cattle industry. Feline trichomoniasis is a colorectal disease that causes chronic diarrhea in domestic cats. In addition, bovine trichomoniasis is a sexually transmitted disease, while feline trichomoniasis is transmitted through the fecal-oral route. Therefore, the diagnosis and treatment of trichomoniasis is of great significance to animal health management.
Figure 1. Fine structure of untreated PS T. foetus (Pereira-Neves, et al. 2015).
Tritrichomonas Foetus PCR Kit Development
Currently, the diagnostic methods for T. foetus include direct microscopic examination of fresh feces, fecal culture and molecular biology methods (e.g., PCR). Since T. foetus requires a strict growth environment, and microscopic examination and fecal culture can affect the detection rate of T. foetus due to delayed examination and refrigerated feces, etc. In recent years, PCR techniques have become one of the most widely used methods for laboratory diagnosis of T. foetus. Researchers have developed several PCR methods with sensitivity and specificity for the diagnosis of T. foetus, including RT-qPCR, nested PCR, and real-time PCR, etc.
BioVenic is a provider of biology reagents and kits, which has been committed to the development of animal pathogens diagnostic reagents for many years. We have a professional team with extensive experience in veterinary diagnostic and reagent development, and our R&D team is working hard to develop and optimize PCR kits. We can provide you with a series of customized PCR kits for the detection of T. foetus according to your needs. If you have any needs, please feel free to contact us. We will provide you with high-quality products and services.
Tritrichomonas Foetus PCR Kit We Can Develop
| T. foetus RT-qPCR kit | T. foetus nested PCR kit | T. foetus real-time PCR kit |
| T. foetus conventional PCR kit | T. foetus multiplex PCR Kit | Other T. foetus PCR Kit |
Workflow of Tritrichomonas Foetus PCR Kit Development
| Workflow | Details |
| Cell cultures | Cultivation of T. foetus from cattle foreskin or cattle and cat feces. |
| Primer and probes design | Available sequences of T. foetus were retrieved from GenBank and specific primer/probe sets were designed. |
| Nucleic acid extraction | Extract DNA from cattle foreskin or fresh feces cultures samples. |
| Establishment of PCR conditions | Establishment of thermocycling conditions to conduct amplification and detection through PCR. |
| Reproducibility evaluation of the PCR assay | Evaluation of reproducibility of PCR by comparing the T. foetus positive samples from different sources. |
| Assessment of sensitivity and specificity | PCR kits were tested for specificity and sensitivity with a variety of pathogens that may be present in foreskin or fecal samples. |
Delivery
- A series of PCR kits for T. foetus
- Product quality inspection report
- Other experimental data you need
Our Advantages
- High purity Taq DNA Polymerase
- High reproducibility between tests
- Highly specific detection profile
- Faster results and greater cost savings
References
- Hafez, H. M., et al. "Comparison of the specificity and sensitivity of PCR, nested PCR, and real-time PCR for the diagnosis of histomoniasis." Avian Diseases 49.3 (2005): 366-370.
- Polo, Coral, et al. "Molecular detection of Tritrichomonas foetus in bovine samples: a novel real-time polymerase chain reaction (PCR) assay targeting EF1-alpha-Tf1 and a comparative study of published PCR techniques." Parasitology Research (2022): 1-9.
- Pereira-Neves, Antonio, et al. "Characterisation of 20S proteasome in Tritrichomonas foetus and its role during the cell cycle and transformation into endoflagellar form." PloS One 10.6 (2015): e0129165.
