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Avibacterium paragallinarum (historically called Hemophilus paragallinarum), is the primary pathogen of infectious coryza (IC) in chickens, which is an acute respiratory disease. A. paragallinarum is a fastidious gram-negative, non-motile, pleomorphic bacterium of the Pasteurellaceae family, which can be divided into three serovars: A, B and C. Several factors are responsible for the pathogenicity of A. paragallinarum. Among them, hemagglutinin (HA) has been regarded as most key virulent factor because of its participation in tissue adhesion. Typical clinical symptoms of IC include ocular discharge, sinusitis and rhinitis, "swollen head" and anorexia have also been reported. IC affects chickens worldwide because it is highly contagious, resulting in poor growth in young birds and reduced egg production in laying hens. The main preventive strategies are management and sound biosecurity measures combined with appropriate vaccination. Outbreaks of IC have been reported in broilers, layer pullets and laying hens, causing significant production losses.
Figure 1. Clinical symptoms of chickens infected with A. paragallinarum (Guo, et al. 2022).
Avibacterium paragallinarum PCR Kit Development
Because of its fastidious nature, A. paragallinarum is difficult to recover and identify, particularly from locations colonized by normal bacterial flora. However, with the development of the biotechnology, A. paragallinarum can be detected through conventional culture examination, post-mortem examination, biochemical identification, conventional PCR and real-time PCR. For preventing the economic losses associated with the IC, an early, accurate and rapid diagnosis is essential. PCR is a rapid and highly sensitive diagnostic technique which can substitute conventional cultural examination.
BioVenic is a provider of biology reagents and kits, which has been committed to the development of animal virus diagnostic reagents for many years. We have a professional team with extensive experience in veterinary diagnostic and reagent development, and our R&D team is working hard to develop and improve PCR kits. We can provide you with a series of customized PCR kits for the detection of Avibacterium paragallinarum according to your needs. If you have any needs, please feel free to contact us. We will provide you with high-quality products and services.
Avibacterium paragallinarum PCR Kit We Can Develop
| A. paragallinarum probe-based PCR kit | A. paragallinarum multiplex real-time PCR kit | A. paragallinarum TaqMan real-time PCR kit |
| A. paragallinarum real-time PCR kit | A. paragallinarum PCR-PFLP kit | A. paragallinarum qPCR kit |
Workflow of Avibacterium paragallinarum PCR Kit Development
| Workflow | Details |
| Real-time PCR assay design | We design specific primers and probe of A. paragallinarum using professional software and platform. |
| Establish detection limit of the PCR | Synthetic DNA fragments are used to avoid the effects of copy number differences in different strains. |
| Genomic DNA preparation | Culture the A. paragallinarum and extract DNA with purified colonies. |
| Real-time PCR | All samples run in triplicates, and the experiment repeats three times. |
| Specificity analysis | Independently validate the specificity analysis by interlaboratory comparison tests. |
Delivery
- A series of RT-PCR kits for Avibacterium paragallinarum
- Product quality inspection report
- Other experimental data you need
Our Advantages
- High sensitivity and specificity
- High repeatability between tests
- Compatible with large number of samples
- Reasonable price and short turnaround time
- Simple and rapid workflow
References
- Guo, Mengjiao, et al. "Pathogenicity and innate response to Avibacterium paragallinarum in chickens." Poultry Science 101.1 (2022): 101523.
- Blackall, Patrick J., et al. "Reclassification of Pasteurella gallinarum, [Haemophilus] paragallinarum, Pasteurella avium and Pasteurella volantium as Avibacterium gallinarum gen. nov., comb. nov., Avibacterium paragallinarum comb. nov., Avibacterium avium comb. nov. and Avibacterium volantium comb. nov." International Journal of Systematic and Evolutionary Microbiology 55.1 (2005): 353-362.
- Kume, K., et al. "Serological classification of Haemophilus paragallinarum with a hemagglutinin system." Journal of Clinical Microbiology 17.6 (1983): 958-964.
