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Neospora caninum is a parasitic protozoan belonging to the class Sporozoa and the genus Neospora, parasitic on cattle, sheep, dogs and other animals. Canines (domestic dogs, mountain dogs, wolves, grey wolves and wild dogs) are the ultimate hosts of N. caninum. Neosporosis is a disease caused by N. caninum that is widespread worldwide and primarily causes abortion or stillbirth in pregnant animals as well as motility disorders and neurological disorders in newborns. N. caninum is a major cause of abortion in livestock worldwide and causes serious economic losses to the farming industry. Therefore, the establishment of an effective method to detect the infection of N. caninum plays an important role in the prevention and control of neosporosis.
Figure 1. Life cycle of Neospora caninum (Dubey, et al.2003).
Neospora caninum ELISA Kit Development
Currently, a variety of serological methods have been developed for the study of N. caninum in animals, including direct agglutination, indirect fluorescent antibody test (IFAT), Western blot and enzyme-linked immunosorbent assay (ELISA). ELISA is a commonly used method for serological detection, which detects N. caninum by specific binding of antigens to antibodies to be tested in serum. This method has the advantages of simple operation, short reaction time and high detection throughput, and is widely used in large-scale screening of animal neosporosis.
BioVenic is a provider of biology reagents & kits, which has been committed to the development of animal pathogen diagnostic reagents for many years. We have a professional team with extensive experience in protein expression and purification and antibody preparation. We can prepare antibodies specific for dense granule antigen and stage-specific proteins of N. caninum, and we can provide you with a range of customized ELISA kits for N. caninum detection according to your needs. If you have any needs, please feel free to contact us. We will provide you with high-quality products for diagnosis of animal diseases.
The ELISA Kits We Can Develop
| N. caninum NcGRA1 ELISA kit | N. caninum NcGRA2 ELISA kit | N. caninum NcGRA6 ELISA kit |
| N. caninum NcGRA7 ELISA kit | N. caninum NcSAG1 ELISA kit | N. caninum NcSAG4 ELISA kit |
| N. caninum NcSRS2 ELISA kit | N. caninum NTPase ELISA kit | Other N. caninum ELISA kit |
Workflow of ELISA Kit Development
| Workflow | Details |
| Submit ELISA kit development requests | Determine the ELISA development protocol and estimate the cost and cycle based on the assay targets and experimental requirements. |
| Antigen preparation | Design and synthesize primers for the nucleic acid sequence encoding the antigenic protein and obtain the target fragment by amplification, connect the target fragment to the expression vector, induce expression to obtain the recombinant protein, and carry out SDS-PAGE and Western blot to identify its immunogenicity, purification recombinant protein. |
| Antibody preparation | Preparation of antibodies specific for dense granule antigen and stage-specific proteins of N. caninum, analysis for antibodies specificity and epitope. |
| ELISA kit development | Optimizing the final reaction conditions, evaluation of sensitivity, specificity and concordance of ELISA kit. |
| ELISA kit delivery | We will provide you with customized ELISA kits and product quality inspection report. |
Delivery
A series of ELISA kits for detection N. caninum
Product quality inspection report (COA)
Other experimental data you need
Our Advantages
- Sensitive and specific detection of pathogen-specific antibodies in animal samples
- Reliable and rapid screening and detection of seropositive animals
- Each kit is rigorously validated and tested
- Reasonable price and short turnaround time
References
- Dubey, John P. "Review of Neospora caninum and neosporosis in animals." The Korean Journal of Parasitology 41.1 (2003): 1.
- Hamidinejat, Hossein, et al. "Development of an indirect ELISA using different fragments of recombinant Ncgra7 for detection of Neospora caninum infection in cattle and water buffalo." Iranian Journal of Parasitology 10.1 (2015): 69.
- Borsuk, Sibele, et al. "Development of an indirect ELISA-NcSRS2 for detection of Neospora caninum antibodies in cattle." Veterinary Parasitology 177.1-2 (2011): 33-38.
