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The intestine may be involved as part of generalized viral infections in disorders such as distemper in dogs. Viral enteritis and diarrhea in dogs have been linked to parvoviruses, coronaviruses, and rotaviruses. Numerous additional viruses with unknown importance and enteropathogenicity have also been discovered in the intestines or excretions of dogs. Herpesvirus, enteroviruses, calicivirus, parainfluenza virus, adenovirus, and picornavirus are a few of them. Since the late 1970s, canine parvovirus type 2 (CPV-2), a non-enveloped, single-stranded DNA virus, has been widely distributed around the world and causes acute, highly contagious canine enteritis. The fecal-oral pathway is how canine parvovirus (CPV) infections happen. Though common, it's challenging to manage canine gastrointestinal infections.
Figure 1. General structure of canine parovirus (Sykes, et al., 2014).
Canine Gastrointestinal Virus VN Kit Development
All canines are susceptible to infection, but some breeds are more likely to contract the parvovirus and tend to be more prone to a severe variant of gastrointestinal illness. The intestinal mucosal barrier breaks down as a result of CPV-induced intestinal epithelial damage. This enables endotoxin absorption and bacterial translocation. The viral neutralization (VN) test has been frequently utilized to identify subgroup-specific antibodies against gastrointestinal diseases in dogs. Neutralization test are built on the concept that when antibodies bind to toxins or pathogens, their biological functions are inhibited. This technique can be used to find antibodies, detect diseases (with specific immunosera), or their toxins (with defined pathogens). The ability to precisely assess serum samples is a benefit of virus neutralization techniques. These days, VN technologies like the plaque reduction neutralization test and the serum virus neutralization (SVN) assay are used to find antibodies.
BioVenic is a supplier of biological reagents and kits, we devoted a lot of time to developing diagnostic tools and kits for animal diseases. Our assays are developed and processed with higher standards and the results are delivered on time without compromising quality. We have a team with significant expertise in veterinary diagnostic, and we will provide a range of specialized viral neutralization (VN) kits for the diagnosis of canine gastrointestinal virus. If you have any needs, please feel free to contact us.
Canine Gastrointestinal Virus VN Kits We Can Provide
| Canine parovirus type 2 VN kit | Canine enterovirus VN kit | Adenovirus VN kit |
| Canine calcivirus VN kit | Other VN kits you need |
Workflow of Canine Gastrointestinal Virus VN Kit Development
| Workflow | Details |
| Virus titration | Stock virus was diluted to its working concentration, and stored at a particular temperature. |
| Dilution | The stock virus was carried to dilution in a virus diluent that contains trypsin, and quadruplicate titrations were carried out. |
| Incubation | Incubation was carried out using a lower supply of carbon dioxide at a particular range temperature. |
| Cell-fixation | The media was carried out of the viral titration. |
| Micro-neutralization assay | Before being utilized in the test, all serum was treated with a receptor-destroying enzyme (RDE) and kept at a specified range of temperature for a set amount of time. |
Delivery
- A series of VN kits for canine gastrointestinal viruses
- Report on product inspection quality
- Other experimental data you need
Our Advantages
- High specificity and sensitivity
- Viral pathogens or their toxins can be detected
- Reasonable price
- Detect the level of serological cross reactivity
References
- Sykes, Jane E. "Canine parvovirus infections and other viral enteritides." Canine and feline infectious diseases (2014): 141.
- Perera, Ranawaka APM, et al., "Evaluation of a SARS-CoV-2 surrogate virus neutralization test for detection of antibody in human, canine, cat, and hamster sera." Journal of clinical microbiology 59.2 (2021): e02504-20.
