Services
Feline respiratory disease complex (FRDC) refers to the acute disease of a contagious respiratory or ocular disease caused by one or multiple pathogens. FRDC is typically characterized by clinical signs of rhinosinusitis, sneezing, conjunctivitis, lacrimation, salivation, and oral ulcerations and fever. The principal pathogens that cause FRDC are feline herpesvirus type 1 (FVR) and feline calicivirus (FCV), while the most common bacteria that cause respiratory infections in cats are Bordetella bronchiseptica and Chlamydophila felis. Diagnosis of a respiratory infection is typically based on the characteristic clinical signs. However, due to the clinical symptoms caused by many different pathogens are similar, the identification of pathogens has become very critical in the prevention and treatment of feline respiratory infections.
Figure 1. Immunohistochemistry findings in cats infected with influenza A(H7N2) virus (Hatta et al., 2018).
Feline Respiratory Pathogens AGID Kit Development
Although there are vaccines that provide protection against specific pathogens, it's still crucial to develop a rapid, simple and economical method for the detection of feline respiratory pathogens to efficiently prevent and control the spread of feline respiratory pathogens. Serological methods and molecular biological methods are usually used to identify equine gastrointestinal pathogens in the laboratory. Agar gel immunodiffusion (AGID), as a simple and convenient serological method in veterinary diagnosis, is very useful for the detection of feline respiratory pathogens. Several AGID techniques have been developed, including radial immunodiffusion, single immunodiffusion assay, double immunodiffusion assay, etc.
BioVenic is a professional biotechnology company and has been focus on the development of animal diseases diagnostic reagents and kits for many years. In the development and optimization of agar gel immunodiffusion kit, our R&D team has conducted a lot of research and can provide you with professional and one-stop services. We can develop a variety of agar gel immunodiffusion kits for the identification of feline respiratory pathogens according to your research. If you have any needs, please feel free to contact us. We will provide you with high-quality products and services.
Feline Respiratory Pathogens AGID Kits We Can Develop
| B. bronchiseptica radial immunodiffusion enzyme assay kit | B. bronchiseptica double immunodiffusion assay kit | C. felis radial immunodiffusion enzyme assay kit |
| C. felis double immunodiffusion assay kit | FVR radial immunodiffusion enzyme assay kit | FVR double immunodiffusion assay kit |
| FCV radial immunodiffusion enzyme assay kit | FCV double immunodiffusion assay kit | Other AGID kits you need |
Workflow of Equine Gastrointestinal Pathogens AGID Kit Development
| Workflow | Details |
| Serum sample collection | Blood was collected from sick cats and left to clot at a specific temperature. |
| Gel preparation | Agarose gel was prepared in a specific buffered saline solution at a specific temperature for a given period. |
| Preparation of agar plates | Dispense the melted agar onto a flat surface and prepare the agar plate at a specific temperature. |
| Agar gel immunodiffusion for antibody detection | For each batch of specimens, a strong positive, weak positive, and negative reference serum will be included to aid in interpreting results. |
| Interpretation of test results for antibody detection | Interpret the result via different analysis methods and evaluate the efficiency of the assay. |
Delivery
- A series of AGID kits for feline respiratory pathogens
- Product quality inspection report
- Other experimental data you need
Our Advantages
- High test-to-test repeatability
- Detects very minute quantity of feline respiratory pathogens with high sensitivity
- Detection of therapeutic pathogens to a level of higher specificity
- Reasonable price and short turnaround time
- Combine AGID with other detection methods to avoid false positives
References
- Hatta, Masato, et al. "Characterization of a feline influenza A (H7N2) virus." Emerging Infectious Diseases 24.1 (2018): 75.
- Barrs, V. R., et al. "Detection of Aspergillus-specific antibodies by agar gel double immunodiffusion and IgG ELISA in feline upper respiratory tract aspergillosis." The Veterinary Journal 203.3 (2015): 285-289.
