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In 1978, avian metapneumovirus (aMPV) was first detected in South Africa, and other countries subsequently reported the detection of aMPV. It is an icosahedral enveloped RNA virus belonging to the family Pneumoviridae, the genus Metapneumovirus. aMPV is divided into four subtypes, A, B, C and D. It has a single-stranded negative-sense RNA genome of about 15 kb and contains 8 genes that encode 9 proteins. aMPV causes respiratory diseases in poultry, especially in turkeys, chickens and ducks, causing significant economic losses to the poultry farming. Therefore, the rapid diagnosis of aMPV is very important for the prevention and treatment of respiratory diseases in poultry.
Figure 1. Spread of aMPV in turkey flocks (1: external sources; 2: direct transmission between birds; 3: Indirect transmission; 4: possible vertical transmission has been reported) (Kaboudi, K., et al. 2021).
Avian Metapneumovirus ELISA Kit Development
aMPV was earlier known as avian pneumovirus, turkeys and chickens are the natural hosts of this virus. aMPV is highly infectious and can infect poultry of different ages and cause a range of associated symptoms. However, aMPV infection cannot be confirmed by symptoms, but by various biological methods, such as virus isolation, immunofluorescence assay (IFA), immunohistochemistry (IHC), reverse transcriptase polymerase chain reaction (RT-PCR), enzyme-linked immunosorbent assay (ELISA), etc. Among these methods, serological methods facilitate large-scale screening and monitoring of flocks. ELISA is a convenient and efficient tool for the detection of aMPV antibodies in poultry as well as other bird species.
BioVenic is a provider of biology reagents & kits, which has been committed to the development of animal virus diagnostic reagents for many years. We have a professional team with extensive experience in protein expression and purification and antibody preparation. We can prepare specific antibodies against the antigens of avian metapneumovirus and can provide you with a series of customized ELISA kits for the detection of avian metapneumovirus according to your needs. If you have any needs, please feel free to contact us. We will provide you with high-quality products for animal diagnostics.
The ELISA Kits We Can Develop
| Chicken avian metapneumovirus ELISA kit | Duck avian metapneumovirus ELISA kit |
| Turkey avian metapneumovirus ELISA kit | Pigeon avian metapneumovirus ELISA kit |
Workflow of ELISA Kit Development
| Workflow | Details |
|---|---|
| Submit ELISA kit development requests | Determine the ELISA development protocol and estimate the cost and cycle based on the assay targets and experimental requirements. |
| Antigen preparation | The avian metapneumovirus antigens were comprehensively analyzed and evaluated to determine the expression intervals of the antigens, and then recombinant antigens with a purity of not less than 90% were prepared for immunization. |
| Antibody preparation | Preparation of antibodies specific for avian metapneumovirus antigens. |
| ELISA kit development | Antibody label, antibody paired screening, method development and optimization, kit production. |
| ELISA kit delivery | We will provide you with customized ELISA kits and complete product quality inspection report. |
Delivery
- A series of ELISA kits for detection avian metapneumovirus
- Product quality inspection report (COA)
- Other experimental data you need
Our Advantages
- High sensitivity and specificity
- High repeatability between tests
- Professional antibody platform to support ELISA kit development
- Reasonable price and short turnaround time
References
- Kaboudi, K., and J. Lachheb. "Avian metapneumovirus infection in turkeys: a review on turkey rhinotracheitis." Journal of Applied Poultry Research 30.4 (2021): 100211.
- Xu, Wanhong, et al. "Development of A recombinant nucleocapsid based indirect ELISA for the detection of antibodies to avian metapneumovirus subtypes, A, B, and C." Veterinary Immunology and Immunopathology 231 (2021): 110151.
