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Canine influenza virus (CIV) is a pathogen of acute respiratory tract infection in dogs and is primarily transmitted to dogs across species from influenza viruses that infect humans, avian and equine. Currently, three major CIV strains have been identified, namely seasonal human-origin H3N2 (hH3N2), avian-origin canine H3N2 (cH3N2) and equine-origin H3N8. Symptoms of canine infection with different subtypes of CIV are similar, mainly including cough, low-grade fever and runny nose. CIV is widely spread and different subtypes of CIV may recombine in the host to generate new CIV, which poses a great threat to human and animal health. Therefore, it is necessary to develop a rapid and accurate detection method to identify different CIV subtypes.
Figure 1. Genetic analysis of influenza viral RNA isolated from infected dogs during the initial influenza outbreak showed that the virus shared >96% of genes with equine-adapted H3N8 (Amber Graham, Kate E. Creevy, 2019).
Canine Influenza Virus PCR Kit Development
Accurate diagnosis of CIV is critical to the treatment and control of canine disease. At present, the diagnostic methods of CIV mainly include virus isolation, serological detection and PCR. However, the virus isolation process is time-consuming and cumbersome, and the sensitivity of serological detection is relatively low. Compared with other CIV diagnostic methods, PCR has the advantages of high sensitivity, high specificity and rapid turnaround time. It is a common method for laboratory CIV diagnosis. Researchers have developed a variety of PCR methods for diagnosis of CIV, including RT-PCR, quantitative real-time PCR, multiplex RT-PCR, iiRT-PCR, etc.
BioVenic is a provider of biology reagents and kits, which has been committed to the development of animal pathogens diagnostic reagents for many years. We have a professional team with extensive experience in veterinary diagnostic and reagent development, and our R&D team is working hard to develop and optimize PCR kits. We can provide you with a series of customized PCR kits for the detection of canine influenza virus according to your needs. If you have any needs, please feel free to contact us. We will provide you with high-quality products and services.
Canine Influenza Virus PCR Kit We Can Develop
| H3N8 RT-PCR kit | H3N8 multiplex RT-PCR kit | H3N8 iiRT-PCR kit |
| H3N8 real-time qPCR kit | cH3N2 RT-PCR kit | cH3N2 multiplex RT-PCR kit |
| cH3N2 iiRT-PCR kit | cH3N2 real-time qPCR kit | hH3N2 RT-PCR kit |
| hH3N2 multiplex RT-PCR kit | hH3N2 iiRT-PCR kit | hH3N2 real-time qPCR kit |
Workflow of Canine Influenza Virus PCR Kit Development
| Workflow | Details |
| Virus and cell cultures | Different viruses were obtained from naturally infected dog, and canine influenza viruses strains were grown in dog cells. |
| Primer and probes design | Design primers or probes to detect H3N8, hH3N2 and cH3N2, and use professional software to select and evaluate primers and probes. |
| Nucleic acid extraction | Extract RNA from nasopharyngeal swabs of infected dogs. |
| Establishment of PCR conditions | Establishment of thermocycling conditions to conduct amplification and detection through PCR. |
| Reproducibility evaluation of the PCR assay | The reproducibility of the PCR kit was assessed by determining the intra-assay and inter-assay coefficients of variation (CV). |
| Assessment of sensitivity and specificity | The specificity of PCR kits was assessed using H3N8, hH3N2 and cH3N2 strains alone or in combination, and detection limits were determined by analyzing 10-fold dilutions of RNA templates extracted from these three strains. |
Delivery
- A series of PCR kits for canine influenza virus
- Product quality inspection report
- Other experimental data you need
Our Advantages
- Highly specific detection profile
- Ready-to-use master mix
- Confidence in results
- Closed tube operation reduces cross-contamination
- Professional R&D personnel and technology platform
References
- Pecoraro, Heidi L., et al. "Evaluation of virus isolation, one-step real-time reverse transcription polymerase chain reaction assay, and two rapid influenza diagnostic tests for detecting canine Influenza A virus H3N8 shedding in dogs." Journal of Veterinary Diagnostic Investigation 25.3 (2013): 402-406.
- Wang, Chenxi, et al. "A multiplex RT-PCR assay for detection and differentiation of avian-origin canine H3N2, equine-origin H3N8, human-origin H3N2, and H1N1/2009 canine influenza viruses." PLoS One 12.1 (2017): e0170374.
- Amber Graham, Kate E. Creevy. "Canine influenza: new strains and treatment."
