Services
Canine infectious respiratory disease complex (CIRDC) is very common disease and can be due to one or more bacterial and viral organisms. The signs of disease can be mild, e.g., cough, sneeze, discharge from the eyes or nose, fever, or severe, such as pneumonia complicated with bacterial infection. In most dogs, signs of disease are mild and self-limiting (i.e., resolve on their own) in 7-10 days. The principal bacteria that cause CIRDC are Streptococcus equi subsp. zooepidemicus, Bordetella bronchiseptica, and Mycoplasma cynos. Canine influenza virus, canine parainfluenza virus, canine distemper virus, canine herpesvirus, canine respiratory coronavirus, and canine adenovirus type 2 are some viral pathogens that cause canine respiratory diseases. A clinical diagnosis of CIRDC can typically be made with a history and physical examination. However, an etiologic diagnosis should be pursued in dogs with severe or prolonged clinical signs or in disease outbreak situations.
Figure 1. Section of canine lung (Typical fibrino-suppurative, necrotizing, and hemorrhagic pneumonia due to S. equi subsp. zooepidemicus) (Priestnall et al., 2010).
Canine Respiratory Pathogens AGID Kit Development
The agar gel immunodiffusion assay is used to diagnose canine respiratory disorders caused by several pathogens. It is a diagnostic test that uses the serum to identify antibodies generated in response to infection. Agar gel immunodiffusion (AGID) assays have been frequently utilized to discover canine respiratory disease-specific antibodies. The agar gel immunodiffusion test has the benefit of being able to reliably assess severely hemolyzed and cytotoxic serum samples. Antibodies can now be identified using AGID technologies such as the radial immunodiffusion assay (RID), the double immunodiffusion assay, etc.
BioVenic as a leading supplier of diagnostic reagents and kits for animal diseases research. We have a professional team with extensive experience in veterinary diagnostics, and our research and development team is working hard to develop and improve agar gel immunodiffusion kits. We can design a range of agar gel immunodiffusion kits for the identification of canine respiratory infections based on your requirements. Please do not hesitate to contact us, if you have any needs. We will provide you with high-quality products and services.
Canine Respiratory Pathogens AGID Kits We Can Develop
| Bordetella bronchiseptica radial immunodiffusion enzyme assay kit | Bordetella bronchiseptica double immunodiffusion assay kit | Mycoplasma cynos radial immunodiffusion enzyme assay kit |
| Mycoplasma cynos double immunodiffusion assay kit | Canine influenza virus radial immunodiffusion assay kit | Canine influenza virus double immunodiffusion assay kit |
| Canine parainfluenza virus radial immunodiffusion assay kit | Canine parainfluenza virus double immunodiffusion assay kit | Other AGID kits you need |
Workflow of Canine Respiratory Pathogens AGID Kit Development
| Workflow | Details |
| Preparation of agarose gel | Agarose gel was prepared and heated for a given period. |
| Preparation of reference antigen | Reference antigens were taken from the dogs infected with respiratory pathogens. |
| Preparation of antiserum | Antiserum was prepared by collecting the serum from dogs and serum was examined rigorously for specificity. |
| Examination of sample | Samples were diluted using distilled water and conducted for examination. |
| Pathogenic antigen separation | Antigens of pathogens were separated using centrifugation. |
| Interpretation of test results for antibody detection | Interpret the result via different analysis methods and evaluate the efficiency of the assay. |
Delivery
- A set of AGID kits for canine respiratory pathogens
- Report on product quality
- List of other experimental data you need
Our Advantages
- Short turnaround time and reasonable price
- Excellent diagnostic relative sensitivity and specificity
- High reproducibility between tests
- Combine AGID with other detection methods to avoid false positives
References
- Priestnall, Simon L., et al. "Characterization of pneumonia due to Streptococcus equi subsp. zooepidemicus in dogs." Clinical and Vaccine Immunology 17.11 (2010): 1790-1796.
- Sharun, K., et al. "How close is SARS‐CoV‐2 to canine and feline coronaviruses?." The Journal of Small Animal Practice 61.8 (2020): 523.
