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Avian reovirus (ARV) is a non-enveloped virus with an icosahedral capsid. The genome of ARV is a double-stranded RNA composed of 10 gene fragments, which are divided into three groups according to the electrophoretic mobility: small fragments (S1-S4), medium fragments (M1-M3) and large fragments (L1-L3). ARV can infect a variety of birds, causing diseases such as dwarf syndrome, pericarditis, myocarditis, and viral arthritis/tendonitis. ARV is widely prevalent worldwide and has had a great impact on the poultry farming industry and socio-economics.
Figure 1. Gross and histopathological lesions in ARV infected tendon tissue (Ayalew, et al. 2017).
Avian Reovirus ELISA Kit Development
In recent years, with the development of biotechnology, several methods have been developed for the detection of avian reovirus. Hemagglutination inhibition (HI) is the standard test for detecting antibodies in avian serum, but it is laborious and prone to false positive results. In contrast, the enzyme-linked immunosorbent assay (ELISA) is easier to standardize and more sensitive, which is suitable for detecting high-throughput samples and is an effective serological method to meet the requirements of epidemic prevention. Currently, researchers have developed several ELISA assays that use ARV single structural proteins as antigens to generate antibodies to reduce non-specific responses.
BioVenic is a provider of biology reagents & kits, which has been committed to the development of animal virus diagnostic reagents for many years. We have a professional team with extensive experience in protein expression and purification and antibody preparation. We can prepare specific antibodies against the antigens of avian reovirus and can provide you with a series of customized ELISA kits for the detection of avian reovirus according to your needs. If you have any needs, please feel free to contact us. We will provide you with high-quality products for animal diagnostics.
The ELISA Kits We Can Develop
| Avian reovirus σA-ELISA kit | Avian reovirus σB-ELISA kit |
| Avian reovirus σC-ELISA kit |
Workflow of ELISA Kit Development
| Workflow | Details |
|---|---|
| Submit ELISA kit development requests | Determine the ELISA development protocol and estimate the cost and cycle based on the assay targets and experimental requirements. |
| Antigen preparation | The structural proteins σA, σB and σC of ARV were analyzed comprehensively to determine the expression intervals of these structural proteins, and then suitable expression systems were selected to prepare recombinant antigens with a purity of not less than 90% for immunoassay. |
| Antibody preparation | Preparation of specific antibodies against ARV structural proteins σA, σB and σC. |
| ELISA kit development | Antibody label, antibody paired screening, method development and optimization, kit production. |
| ELISA kit delivery | We will provide you with customized ELISA kits and complete product quality inspection report. |
Delivery
- A series of ELISA kits for detection avian reovirus
- Product quality inspection report (COA)
- Other experimental data you need
Our Advantages
- High specificity and very low background
- High repeatability between tests
- Each kit is rigorously validated and tested
- Professional technology platform
- Reasonable price and short turnaround time
References
- Ayalew, Lisanework E., et al. "Phenotypic, genotypic and antigenic characterization of emerging avian reoviruses isolated from clinical cases of arthritis in broilers in Saskatchewan, Canada." Scientific Reports 7.1 (2017): 1-13.
- Yang, Zi-Jiang, et al. "Development of ELISA kits for antibodies against avian reovirus using the σC and σB proteins expressed in the methyltropic yeast Pichia pastoris." Journal of Virological Methods 163.2 (2010): 169-174.
