Brucella Plate Agglutination Test Kit Development

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Brucella Plate Agglutination Test Kit Development

Brucellaare cocci with a diameter of about 0.6 micrometers or bacilli with a length of more than 1 micrometer. Brucella is a Gram-negative bacterium that causes a variety of mammalian infections. Although Brucella can be divided into 10 species based on diagnostic and epidemiological characteristics, genomic studies have shown that different Brucella are highly similar at the genomic level. The genus Brucella that infects animals mainly includes Brucella abortus, Brucella melitensis, Brucella suis and Brucella canis.

Cycle of Brucella abortus in the bovine host and humansFigure 1. Cycle of Brucella abortus in the bovine host and humans (Moreno, Edgardo, et al. 2020).

Brucella Plate Agglutination Test Kit Development

Brucellosis is a disease caused by bacteria of the genus Brucella that primarily infects cattle, pigs, goats, sheep and dogs. Brucellosis is one of the most widespread zoonotic diseases. In endemic areas, human infection with brucellosis has serious public health consequences. Expansion of animal husbandry, urbanization and lack of hygiene measures in food handling are some of the reasons for the brucellosis epidemic. In recent years, scientists have developed a variety of detection methods for the diagnosis of brucellosis. Plate agglutination test is widely used due to its simple operation and visualization of results.

BioVenic is a supplier of diagnostic reagents for animal diseases and has been working for many years on animal pathogens to improve animal health. We have a professional team and advanced equipment. We can develop a range of Brucella PAT kits for you to rapidly diagnose Brucella infection and control the spread of brucellosis. If you have any needs, please feel free to contact us. We will provide you with high-quality products and services.      

The Brucella Plate Agglutination Test Kits We Can Develop

Sheep B. melitensis PAT kit
Bovine B. abortus PAT kit
Pig B. suis PAT kit

Goat B. melitensis PAT kit
Dog B. canis PAT kit
Other Brucella PAT kit

Workflow of Brucella Plate Agglutination Test Kit Development

Workflow Details
Sample collection Samples were collected from animals infected with Brucella.
Production of seeds Samples were inoculated on agar culture, and after a period of incubation at 37°C, a sample from every single colony was transferred to the broth and incubated at 37°C.
Preparation of chromogenic antigens Neotetrazoles and tetrazolium staining antigens were prepared for use, and the concentration of staining antigens was measured by the Folin Phenol method.
Brucella PAT kit development Development of Brucella plate agglutination test kit using staining antigen.

Delivery

  • A series of Brucella PAT kits
  • Product quality inspection report (COA)
  • Other experimental data you need

Our Advantages

  • High sensitivity and specificity
  • Easy and fast, results are visible to the naked eye
  • Suitable for testing a large number of samples
  • Reasonable price and short turnaround time

Reference

  1. Moreno, Edgardo, and Elías Barquero-Calvo. "The role of neutrophils in brucellosis." Microbiology and Molecular Biology Reviews 84.4 (2020): e00048-20.

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