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Rotavirus is a double-stranded RNA virus of the family Reoviridae. Avian rotavirus (AvRV) was first reported in turkey poults in the USA in 1977 and since then RVs of group A (RVA), D (RVD), F (RVF), and G (RVG) have been identified around the globe. AvRV is usually detected in chickens, turkeys, ducks, guinea fowl, pheasants, partridges, pigeons, and other avian species. The RVA is mainly associated with diarrhea in humans, mammals, and poultry. Besides RVA, other AvRV groups (RVD, RVF, RVG) may also contribute to disease. RVD, RVF, and RVG have been seen exclusively in poultry. The D group has been frequently detected while AvRVs of groups F and G have been identified occasionally. Rotavirus has a great impact on public health and the poultry industry and caused huge economic losses.
Figure 1. Avian rotavirus structure (Crawford, et al. 2017).
Avian Rotavirus PCR Kit Development
Despite the increasing frequency of AvRV infections in poultry worldwide, isolates from poultry have yet to be identified. Sensitive and rapid detection methods for AvRV have been lacking in the past. However, with the development of biotechnology, several polymerase chain reaction (PCR) methods have been established in recent years to detect avian rotavirus, such as reverse transcription PCR (RT-PCR), quantitative PCR (qPCR), real time reverse transcription PCR (rRT-PCR), etc. These PCR methods are widely used in the rapid diagnosis of AvRV infection, monitoring and evaluation of treatment efficacy, and detection of infectious agents due to their high sensitivity, good specificity, fast speed and high efficiency.
BioVenic is a provider of biology reagents and kits, which has been committed to the development of animal virus diagnostic reagents for many years. We have a professional team with extensive experience in veterinary diagnostic and reagent development, and our R&D team is working hard to develop and improve PCR kits. We can provide you with a series of customized PCR kits for the detection of avian rotavirus according to your needs. If you have any needs, please feel free to contact us. We will provide you with high-quality products and services.
Avian Rotavirus PCR Kit We Can Develop
| RVA RT-PCR kit | RVA qPCR kit | RVA rRT-PCR kit |
| RVD RT-PCR kit | RVD qPCR kit | RVD rRT-PCR kit |
| RVF RT-PCR kit | RVF qPCR kit | RVF rRT-PCR kit |
| RVG RT-PCR kit | RVG qPCR kit | RVG rRT-PCR kit |
Workflow of PCR Kit Development
| Workflow | Details |
|---|---|
| Viral RNA extraction | Extract the viral RNA from the AvRV-positive sample. |
| RNA-polyacrylamide gel electrophoresis (RNA-PAGE) | For the detection of 11 segmented dsRNA of rotavirus, the extracted RNA is subjected to RNA-PAGE. |
| Preparation of complementary DNA | Synthesis cDNA by reverse transcription from viral RNA for primer designing. |
| Designing of AvRV specific primers | Primer of RVD can be designed using professional software and database. |
| Optimization of RT-PCR assay | We use a partial plasmid (185 bp) of the specific AvRV gene as a template for optimizing and evaluating the sensitivity of the PCR assay. |
| Assessment of PCR efficiency | Independently validate the AvRV PCR assay by interlaboratory comparison tests. |
Delivery
- A series of PCR kits for avian rotavirus
- Product quality inspection report
- Other experimental data you need
Our Advantages
- High sensitivity and specificity
- High repeatability between tests
- Adaptable to high throughput workflows
- Compatible with a large number of samples
- Simple and rapid workflow
References
- Elschner, M., et al. "Isolation, identification and characterization of group A rotavirus from a chicken: the inner capsid protein sequence shows only a distant phylogenetic relationship to most other avian group A rotaviruses." Journal of Veterinary Medicine, Series B 52.5 (2005): 211-213.
- Islam, M. S., et al. "Molecular epidemiologic study on rotavirus infection in human and birds in association with gastroenteritis." Bangladesh Journal of Veterinary Medicine 7.1 (2009): 233-237.
- Crawford, Sue E., et al. "Rotavirus infection." Nature Reviews Disease Primers 3.1 (2017): 1-16.
