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Bovine leukemia virus (BLV) is a C-type virus belonging to the subfamily Oncoviridae of the family Retroviridae, with viral particles 90-120 nm in diameter and a genome length of 8.7 kbp. The genome of BLV including structural and non-structural genes. BLV is widespread in cattle in many countries and causes persistent lymphocytosis in cattle, leading to the development of bovine endemic leukemia. BLV is transmitted mainly through direct contact with the blood of infected cattle and has a significant impact on the health of infected animals and the trade of beef and dairy products. In addition, it has caused significant economic losses to the cattle industry. To reduce the losses caused by BLV, serological testing of cattle and culling of infected animals are needed to eradicate bovine leukemia virus infection.
Figure 1. Schematic representation of the BLV viral particle (Gillet, et al. 2007).
Bovine Leukosis Virus ELISA Kit Development
The genome of BLV encodes matrix protein p15, capsid protein p24, nucleocapsid protein p12, surface glycoprotein p51 and transmembrane protein p30. Following BLV infection, cattle produce antibodies primarily against viral proteins p24 and p51 and elicit a strong humoral immune response. Therefore, BLV can be diagnosed by detecting viral antibodies and viral antigens. In recent years, various serological methods have been developed: such as enzyme-linked immunosorbent assay (ELISA), agar gel immunodiffusion assay (AGID), phytohemagglutinin (PHA) test, etc. Each method differs in sensitivity and specificity, and AGID and PHA methods can produce variable results. In addition, AGID is less sensitive to infected cattle with low antibody titers. ELISA has the advantages of high sensitivity, high specificity and easy standardization as a commonly used serological test for BLV.
BioVenic is a provider of biology reagents & kits, which has been committed to the development of animal virus diagnostic reagents for many years. We have a professional team with extensive experience in protein expression and purification and antibody preparation. We can prepare specific antibodies against the different antigens of bovine leukosis virus and can provide you with a series of customized ELISA kits for the detection of bovine leukosis virus according to your needs. If you have any needs, please feel free to contact us. We will provide you with high-quality products for animal diagnostics.
The ELISA Kits We Can Develop
| Bovine leukemia virus p24-ELISA kit | Bovine leukemia virus competitive p51-ELISA kit |
Workflow of ELISA Kit Development
| Workflow | Details |
|---|---|
| Submit ELISA kit development requests | Determine the ELISA development protocol and estimate the cost and cycle based on the assay targets and experimental requirements. |
| Antigen preparation | DNA extraction, cloning and sequencing of genes encoding the capsid protein p24 and surface glycoprotein p51 of BLV, and then constructing vectors to express p24 and p51 proteins. |
| Antibody preparation | Preparation of antibodies specific for capsid protein p24 and surface glycoprotein p51 of BLV. |
| ELISA kit development | Antibody label, antibody paired screening, method development and optimization, kit production. |
| ELISA kit delivery | We will provide you with customized ELISA kits and complete product quality inspection report. |
Delivery
- A series of ELISA kits for detection bovine leukemia virus
- Product quality inspection report (COA)
- Other experimental data you need
Our Advantages
- High sensitivity and specificity
- Good adsorption performance, low blank value
- High-throughput detection
- Reasonable price and short turnaround time
References
- Bai, Lanlan, et al. "Development of a new recombinant p24 ELISA system for diagnosis of bovine leukemia virus in serum and milk." Archives of Virology 164.1 (2019): 201-211.
- Gillet, Nicolas, et al. "Mechanisms of leukemogenesis induced by bovine leukemia virus: prospects for novel anti-retroviral therapies in human." Retrovirology 4.1 (2007): 1-32.
- Andreolla, Ana Paula, et al. "Development of an indirect ELISA based on recombinant capsid protein to detect antibodies to bovine leukemia virus." Brazilian Journal of Microbiology 49 (2018): 68-75.
