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Pestiviruses are positive-stranded RNA viruses belonging to the Flaviviridae family, and their genomes contain a long open reading frame (ORF) of approximately 4000 codons. Pestiviruses include classical swine fever virus (CSFV), border disease virus (BDV) and bovine viral diarrhea virus (BVDV-1, BVDV-2). Classic swine fever (CSF), a disease caused by pestivirus, was first reported in 1833. In 2017, the last update of the pestivirus taxonomy resulted in the classification of 11 species designated pestivirus A to pestivirus K. Pestivirus that have been reported include pathogens associated with swine or small ruminant disease. In addition, pestivirus sequences have also been found during genomic analysis of non-ungulates such as bats and rodents.
Figure 1. Basic features of pestiviruses. The figure shows a diagram of a pestiviral particle flanked by electron microscopic pictures (BVDV in ultrathin section on the left; CSFV in negative stain on the right). Below, the viral genome is shown in a schematic representation with the single long ORF encoding a polyprotein indicated below. Processing of the polyprotein by the proteases specified at the bottom leads to the shown viral proteins (Tautz, Norbert, et al. 2015).
Pestivirus Isolation Kit Development
Pestivirus mainly infect pigs and ruminants, such as cattle, sheep, goats, etc., and seriously affect the health of these animals and lead to increased mortality. Pigs and domestic ruminants are the main sources of meat and dairy products worldwide, so the spread of pestivirus also affects food security worldwide. Therefore, it is very important to develop method for accurate detection of pestivirus. For the diagnosis of viral diseases, the most reliable method is to isolate and identify the pathogenic virus strain from the sample. Since viruses must proliferate in living cells, cell culture has become a widely used method for virus isolation and identification.
Bluetongue (BT) is a disease that severely affects domestic and wild ruminants, especially sheep, which can lead to reduced milk production, miscarriage, and even death in ruminants. Therefore, the detection of bluetongue virus is crucial for the control and treatment of bluetongue. BioVenic is a company specializing in animal virology research, dedicated to safeguarding the health of poultry, livestock, companion animals and wildlife. With professional technicians and advanced testing equipment, we can provide you with a variety of pestivirus isolation kits for the diagnosis of animal diseases. If you have any needs, please feel free to contact us.
The Pestivirus Isolation Kits We Can Develop
Border disease virus- BD31 isolation kit
Bovine viral diarrhea virus 1 isolation kit
Bovine viral diarrhea virus 1-SD-1 isolation kit
Bovine viral diarrhea virus 2 isolation kit
Bovine viral diarrhea virus 2-C413 isolation kit
Classical swine fever virus -C strain isolation kit
Border disease virus -X818 isolation kit
Bovine viral diarrhea virus 1-NADL isolation kit
Bovine viral diarrhea virus 1-CP7 isolation kit
Bovine viral diarrhea virus 2-890 isolation kit
Classical swine fever virus -Alfort/187 isolation kit
Other pestivirus isolation kit
Workflow of Pestivirus Isolation Kit Development
| Workflow | Details |
|---|---|
| Sample collection | Samples are collected from infected animals. |
| Cell culture | Samples were inoculated onto the culture medium for propagation. |
| RNA isolation and purification | Isolation and purification of pestivirus RNA from cell culture fluid. |
| Structural analysis of pestivirus | The isolated pestivirus acid and protein coat were analyzed. |
| Pestivirus isolation kit development | Development of kit to isolate pestivirus. |
| Pestivirus isolation kit delivery | We will provide you with customized pestivirus isolation kits and complete product quality inspection reports. |
Delivery
- A series of pestivirus isolation kits
- Product quality inspection report (COA)
- Other experimental data you need
Our Advantages
- Accurate and reliable results
- Advanced equipment and professional technicians
- Reasonable price and short turnaround time
References
- Tautz, Norbert, and Heinz-Jürgen Thiel. "Pestivirus NS2-3/NS3 serine peptidase." Handbook of Proteolytic Enzymes (2013): 3133.
- Tautz, Norbert, Birke Andrea Tews, and Gregor Meyers. "The molecular biology of pestiviruses." Advances in Virus Research 93 (2015): 47-160.
