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Mycoplasma gallisepticum (MG) belongs to the genus Mycoplasma as a pathogenic species. It is about 0.25-0.5 μm in size, with only cell membrane wrapped without cell wall. MG is well stained with Giemsa or Wright's staining, which is lavender, and lightly stained with Gram stain, which is weakly negative. When observed under the electron microscope its morphology varies, generally spherical, ovoid, sometimes rod-shaped, ball and rod-shaped. Mycoplasmosis is an infectious disease caused by MG characterized by respiratory lesions such as bronchitis, sinusitis, pneumonia, air sac inflammation and other symptoms.
Figure 1. Histologic section of the trachea of the experimentally infected chickens. a, Nonchallenged chicken. b, Chicken challenged with low pathogenicity avian influenza (LPAI) H3N8 virus. c, Chicken challenged with Mycoplasma gallisepticum. d, Chicken challenged with M. gallisepticum and LPAI H3N8 virus (Miao, Yusong, et al. 2020).
Mycoplasma gallisepticum Plate Agglutination Test Kit Development
Mycoplasma gallisepticum infection is prevalent in chicken farms worldwide. In addition to the pathogenicity of mycoplasma itself, the age of chickens is also a factor that affects the prevalence of MG infection in chickens. The infectivity of chickens to MG increases with age. Concurrent infection has a considerable impact on MG infection, so when flocks have respiratory symptoms, attention should be paid to the differential diagnosis of Newcastle disease, infectious bronchitis, infectious rhinitis, Mycoplasma synoviae infection and chicken cholera. Serological methods are used for the differential diagnosis of MG because of their high specificity and simple operation. Plate agglutination test is one of the commonly used serological methods.
BioVenic is a supplier of diagnostic reagents for animal diseases and has been working for many years on animal pathogens to improve animal health. We have a professional team with extensive working experience in serological diagnosis, bacterial immunology, pathogenic microorganisms and kit development. We can develop a range of Mycoplasma gallisepticum PAT kits for you to rapidly diagnose Mycoplasma gallisepticum infection and control the spread of related diseases. If you have any needs, please feel free to contact us. We will provide you with high-quality products and services.
The Mycoplasma gallisepticum Plate Agglutination Test Kits We Can Develop
Chicken Mycoplasma gallisepticum PAT kit
Duck Mycoplasma gallisepticum PAT kit
Pigeon Mycoplasma gallisepticum PAT kit
Turkey Mycoplasma gallisepticum PAT kit
Goose Mycoplasma gallisepticum PAT kit
Other Mycoplasma gallisepticum PAT kit
Workflow of Mycoplasma gallisepticum Plate Agglutination Test Kit Development
| Workflow | Details |
|---|---|
| Sample collection | Samples were collected from animals infected with Mycoplasma gallisepticum. |
| Production of seeds | Samples were inoculated on Mycoplasma agar, and after a period of incubation at 37°C, a sample from each single colony was transferred to Mycoplasma broth and incubated at 37°C. |
| Preparation of chromogenic antigens | Neotetrazoles and tetrazolium staining antigens were prepared for use, and the concentration of staining antigens was measured by the Folin Phenol method. |
| Mycoplasma gallisepticum PAT kit development | Development of Mycoplasma gallisepticum plate agglutination test kit using staining antigen. |
Delivery
- A series of Mycoplasma gallisepticum PAT kits
- Product quality inspection report (COA)
- Other experimental data you need
Our Advantages
- High sensitivity and specificity
- Easy and fast, results are visible to the naked eye
- Suitable for testing a large number of samples
- Reasonable price and short turnaround time
References
- Miao, Yusong, et al. "Mycoplasma gallisepticum induced inflammation-mediated Th1/Th2 immune imbalance via JAK/STAT signaling pathway in chicken trachea: Involvement of respiratory microbiota." Veterinary Microbiology 265 (2022): 109330.
- Wyrzykowski, B., et al. "Retrospective study of Mycoplasma gallisepticum meningoencephalitis in six turkey flocks in western France." Journal of Comparative Pathology 148.2-3 (2013): 173-177.
