There are many types of common gastrointestinal diseases in poultry, which are mainly reflected in decreased feed intake and watery stool. The mild symptoms include catarrhal enteritis, decreased intestinal digestibility, diarrhea, dehydration, fear of cold, reduced food intake, etc. The severe symptoms include increased intestinal mucus secretion, mucosal cell drop, intestinal bleeding, etc. These infections affect birds of all ages but usually predominate in young birds. Many viral pathogens have been identified as the cause of gastrointestinal tract infections in poultry, including rotavirus, coronavirus, enterovirus, adenovirus, astrovirus, and reovirus, and they likely contribute to the development of other extragastrointestinal diseases. The outbreak of avian gastrointestinal disease brings huge losses to global poultry production every year. Elimination of pathogenic factors is the most important method to prevent avian gastrointestinal diseases.
Figure 1. Schematic representation of rotavirus structure (Uprety, et al., 2021).
An understanding of the viruses that cause gastrointestinal tract disease in poultry is necessary for the development of appropriate control procedures. Since avian gastrointestinal disorders are caused by the interaction of different pathogens with birds, methods such as pathogen isolation and clinical symptoms are unable to accurately identify pathogens. To successfully stop the spread the avian gastrointestinal diseases and reduce financial losses, it is crucial to establish a precise, efficient and thorough method for identifying the avian gastrointestinal virus. In recent years, the avian gastrointestinal virus has been diagnosed in the lab using both serological and molecular biology techniques. When compared to other laboratory techniques, the virus neutralization assay outperforms them in speed, sensitivity, and specificity. It looks for antibodies that can neutralize virus infection and can be helpful to stop the spread of viruses. The VN test also constitutes the gold standard test for serological virus identification.
BioVenic is a supplier of biological reagents and kits, and has been developing diagnostic reagents for animal viruses for many years. Our goal is to be a great company that improves the health and well-being of pets and livestock. We have a professional team with extensive experience in veterinary diagnostics and reagent development, and we can provide you with specialized avian gastrointestinal virus VN kits based on your needs. If you have any queries, please feel free to contact us. We will provide you with high-quality products and services.
Avian rotavirus VN kit assay | Avian coronavirus VN kit assay | Avian enterovirus VN kit assay |
Avian adenovirus VN kit assay | Other VN kits you need |
Workflow | Details |
Serum preparation | Two-fold serial dilution of samples was prepared thrice. |
Working suspension | Fifty microlitres of working suspension were prepared. |
Incubation | Plates were incubated for an hour at a specific temperature. |
Mixing of serum-virus | 100 microlitres of the serum-virus mixture was added and plated were again incubated. |
Record of titer | Neutralizing antibody titer was recorded as the reciprocal of the highest serum dilution. |
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