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Anaplasma marginale (Rickettsiales: Anaplasmataceae) is an intracellular pathogenic gram-negative stain microorganism with an outer membrane composed of lipopolysaccharides. which is endemic worldwide in tropical and subtropical areas. Infection of cattle with Anaplasma marginale leads to bovine anaplasmosis, a mild to severe hemolytic disease that causes significant economic losses to the dairy and beef industries. Biologically, Anaplasma marginale is transmitted to cattle by ticks, mechanically through the bite of flies and by phosphorus mites contaminated with blood. Both male ticks and bovine hosts could be persistently infected with Anaplasma marginale and become reservoirs of infection.
Figure 1. Proposed life cycle of Anaplasma marginale (Rodríguez et al., 2009).
Anaplasma marginale PCR Kit Development
Despite the fact that anaplasmosis caused by Anaplasma marginale is one of the most common diseases in grazing animals worldwide, there was no rapid and effective test for detecting species anaplasmosis in the past decades. The method most often used to diagnose Anaplasma marginale is the direct examination on a blood smear, in which sensitivity and specificity are limited compared to the PCR technique. PCR methods that have been developed to detect Anaplasma marginale in recent years include RT-PCR, real-time PCR, multiplex RT-PCR kit, multiplex rRT-PCR, nested PCR kit, RT-qPCR, etc. These specific PCR subtypes are widely used because of their excellent sensitivity and efficiency, which also provide additional opportunities for the development of new strategies for the control and prevention of Anaplasma marginale-related bovine anaplasmosis.
BioVenic is a provider of biology reagents and kits, which has been committed to the development of animal virus diagnostic reagents for many years. We have a professional team with extensive experience in veterinary diagnostic and reagent development, and our R&D team is working hard to develop and improve PCR kits. We can provide you with a series of customized PCR kits for the detection of Anaplasma marginale according to your needs. If you have any needs, please feel free to contact us. We will provide you with high-quality products and services.
Anaplasma marginale PCR Kit We Can Develop
| A. marginale RT-PCR kit | A. marginale real-time PCR kit | A. marginale multiplex RT-PCR kit |
| A. marginale duplex rRT-PCR kit | A. marginale nested PCR kit | A. marginale RT-qPCR kit |
Workflow of Anaplasma marginale PCR Kit Development
| Workflow | Details |
| DNA extraction | Genomic DNA could be extracted and purified from blood samples from A. marginale infected bovine. |
| Design of PCR primers | A set of primers can be designed using professional software. |
| PCR conditions optimization | Maintenance of thermocycling conditions to assure the best ratio between specificity and sensitivity and to conduct amplification and detection through PCR. |
| PCR amplification | Once optimized, PCR would be used to amplify A. marginale from the samples. |
| Cloning and sequencing | Randomly select PCR products, would be purified by a PCR and gel purification kit, then cloned into a cloning vector, and sent for sequencing strands to confirm the identity of the amplicons for a specific strain of A. marginale. |
| Assessment of sensitivity and specificity | Test negative controls and some other pathogens such as A. phagocytophilum, A. platys, Ehrlichia canis, Rickettsia conorii, Coxiella burnetii, Babesia ovis, B. bovis, B. bigemina, B. canis, B. gibsoni, B. caballi to evaluate the and sensitivity specificity of the assay. |
Delivery
- A series of PCR kits for A. marginale
- Product quality inspection report
- Other experimental data you need
Our Advantages
- Positive copy number standard curve for accurate quantification
- Highly specific detection profile with high efficiency priming
- Each kit is rigorously validated and tested
- Simple and rapid workflow
References
- Rodríguez, Sergio D., et al. "Molecular epidemiology of bovine anaplasmosis with a particular focus in Mexico." Infection, Genetics and Evolution 9.6 (2009): 1092-1101.
- Kocan, Katherine M., et al. "The natural history of Anaplasma marginale." Veterinary parasitology 167.2-4 (2010): 95-107.
- Carelli, G., et al. "Detection and quantification of Anaplasma marginale DNA in blood samples of cattle by real-time PCR." Veterinary Mcrobiology 124.1-2 (2007): 107-114.
