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The most common respiratory infections in horses are equine herpesvirus infection, equine influenza, and equine viral arteritis. Pyrexia, serous nasal discharge, submandibular lymphadenopathy, anorexia, and cough are all clinical manifestations. Equine viral arteritis produces respiratory disease, vasculitis, and abortion. Equine herpesvirus type 1 (EHV-1), equine herpesvirus type 2 (EHV-2), equine rhinitis virus and reovirus are common viral respiratory pathogens that cause mild clinical disease. In Arabian foals, adenovirus pneumonia is most associated with severe combined immunodeficiency. Because viral respiratory infections impair and destroy respiratory defense mechanisms, secondary bacterial respiratory infections are primarily the result of viral disease. Mucopurulent nasal discharge, depression, persistent fever, abnormal lung sounds, hyperfibrinogenemia, and leukocytosis are all symptoms of a secondary bacterial infection. Viral equine respiratory tract infections cause huge economic losses to the world horse industry every year.
Figure 1. Schematic illustration of EHV-1 Structure (Oladunni et al., 2019).
Equine Respiratory Virus VN Kit Development
The most widely used method to control viral equine respiratory tract infection is vaccination. Duration and severity are usually lessened in horses that have been vaccinated regularly, depending on factors such as the disease and specific vaccine. However, vaccination does not always prevent respiratory infections in horses. To prevent and control viral equine respiratory tract infections, detection and identification of the viruses are essential. Therefore, an accurate, rapid, and comprehensive method for detecting equine respiratory virus must be developed. In recent years, serological and molecular biological methods have been used in the laboratory to diagnose the equine respiratory virus. When compared to biological methods (e.g., PCR), virus neutralization (VN) has significant advantages in terms of speed, sensitivity, and specificity. Several virus neutralization (VN) methods for detecting equine respiratory pathogens such as viruses and bacteria have been developed by researchers.
BioVenic is a supplier of biological reagents and kits, and has been developing diagnostic reagents for animal viruses for many years. Our goal is to be a great company that improves the health and well-being of pets and livestock. We have a professional team with extensive experience in veterinary diagnostics and reagent development, and we can provide you with a series of customized virus neutralization kits for the detection of equine respiratory virus based on your needs. If you have any queries, please feel free to contact us. We will provide you with high-quality products and services.
Equine Respiratory Virus VN Kit We Can Develop
| Equine herpesvirus VN kit | Equine influenza VN kit | Equine viral arteritis VN kit |
| Other VN kits you need |
Workflow of Equine Respiratory Virus VN Kit Development
| Workflow | Details |
| Viral source | The identified equine herpesvirus strains were acquired. |
| Antibody source | Two milliliters of monoclonal antisera against each strain of the virus were prepared. |
| Optimization process | Variables were optimized during the study for the best neutralization of homologous antigens and antibodies under controlled conditions. Each of the parameters was tested five times in every experiment. |
| Inoculation | Antigen and antiserum suspension was centrifuged at 3600g for five minutes. |
| Controlling parameters | Five serum samples were obtained from non-exposed virus vaccinates. The samples were evaluated for relevant virus exposure through PCR and HI tests using homologous antigens. |
| Evaluation of process | Specificity and sensitivity were evaluated by considering a series of parameters. |
Delivery
- A series of VN kits for equine respiratory pathogens
- Product quality inspection report
- Other experimental data you need
Our Advantages
- High reproducibility between tests
- Effective for detecting the monoclonal antibody
- Capable of quantitively detecting the pathogens
- Reasonable price and short turnaround time
References
- Oladunni, Fatai S., David W. Horohov, and Thomas M. Chambers. "EHV-1: A constant threat to the horse industry." Frontiers in Microbiology 10 (2019): 2668.
- Goodman, Laura B., et al., "Comparison of the efficacy of inactivated combination and modified-live virus vaccines against challenge infection with neuropathogenic equine herpesvirus type 1 (EHV-1)." Vaccine 24.17 (2006): 3636-3645.
