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All the organs involved in ingesting and processing food are a part of the equine gastrointestinal system. It starts in the mouth and extends to the rectum, anus, liver, pancreas, intestines, and esophagus. A horse's excessive drooling, diarrhea, constipation, bleeding, loss of appetite, bloating, and dehydration are all indications of gastrointestinal problems. While viruses rarely affect adult horses, they appear to induce diarrhea in foals. Although other viruses, such as coronavirus, have been suggested as potential causes, rotavirus is the primary cause of viral diarrhea in foals. Rotavirus-caused diarrhea is characterized by depression, anorexia, and copious amounts of watery, foul-smelling excrement. To prevent and early diagnose disorders of the horse gastrointestinal tract, research into equine gastrointestinal viruses is becoming increasingly important.
Figure 1. Aspects of rotavirus structure (Desselberger et al., 2014).
Equine Gastrointestinal Virus VN Kit Development
It is essential to create a quick approach for the identification of equine gastrointestinal viruses to effectively limit the spread of equine gastrointestinal viruses and limit economic losses. In recent years, equine gastrointestinal infections have been diagnosed in the lab using molecular biology and serological techniques. Virus neutralization, as opposed to molecular biological techniques, results in the inhibition of viral attachment to the cell (using fibroblasts as target cells) or inhibition of viral reverse transcription and/or integration (using macrophages as target cells). This method can be applied to detect pathogens (with defined immunosera) or their toxins or to identify antibodies.
BioVenic is a supplier of biological reagents and kits, and has been developing diagnostic reagents for animal viruses for many years. Our goal is to be a great company that improves the health and well-being of pets and livestock. We have a professional team with extensive experience in veterinary diagnostics and reagent development, and we can provide you with a range of customized VN kits for the detection of equine gastrointestinal virus according to your needs. If you have any queries, please feel free to contact us. We will provide you with high-quality products and services.
Equine Gastrointestinal Virus Kit We Can Develop
| G3A equine rotavirus VN kit | G3P equine rotavirus VN kit | Equine coronavirus VN kit |
| Equine coronavirus VN kit | Other VN kits you need |
Workflow of Equine Gastrointestinal Virus VN Kit Development
| Workflow | Details |
| Serum samples preparation | Serial two-fold dilutions of serum samples were prepared in triplicate. |
| Working suspension preparation | Fifty microliters of a working suspension of either the H2 (G3P or RVA) was prepared. |
| Incubation period | Plates were incubated for 1 h at 37 °C in 5% CO2. |
| Mixing of serum-virus | 100 μl of the serum-virus mixture was added to each well, and the plates were incubated at 37 °C in 5% CO2 for 72 h. |
| Titer record | The neutralizing antibody titer was recorded as the reciprocal of the highest serum dilution. |
Delivery
- A series of VN kits for equine gastrointestinal viruses
- Product quality inspection report
- Other experimental data you need
Our Advantages
- High test-to-test repeatability
- Excellent reproducibility and sensitivity
- Therapeutic pathogens to a level of higher specificity can be detected
- Reasonable price and short turnaround time
References
- Desselberger, Ulrich. "Rotaviruses." Virus research 190 (2014): 75-96.
- Carossino, Mariano, et al., "Detection, molecular characterization and phylogenetic analysis of G3P [12] and G14P [12] equine rotavirus strains co-circulating in central Kentucky." Virus Research 255 (2018): 39-54.
