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Diseases of the gastrointestinal tract are a common problem in cats, and vomiting, diarrhea, anorexia and weight loss are the most notable clinical signs associated with these disease. There are many infectious agents associated with gastrointestinal disease in cats, including bacteria (Salmonella spp., Campylobacter jejuni, Clostridium perfringens and Helicobacter spp.), viruses (feline coronaviruses, feline leukemia virus (FeLV) and feline immunodeficiency virus), and parasites (Ancylostoma, Uncinaria, Strongyloides cati, Dirofilaria immitis, etc.). According to reports, feline gastrointestinal diseases account for more than 80% of feline diseases. Therefore, the prevention and control of gastrointestinal diseases in cats is an important aspect of maintaining cat health.
Figure 1. Histopathology of intestinal disorders in cats (da Silva et al., 2018).
Feline Gastrointestinal Pathogens AGID Kit Development
Over the past few decades, serological methods and molecular biological methods have been developed for the diagnosis of feline gastrointestinal diseases. Agar gel immunodiffusion (AGID) technique, as a serological method, is a diagnostic test using serum that detects antibody produced in response to infection. AGID has been widely utilized in detection of feline gastrointestinal pathogens due to its rapidity, simplicity and low cost compared with molecular biological methods. Several types of AGID techniques have been developed, including radial immunodiffusion, single immunodiffusion assay, double immunodiffusion assay, etc.
BioVenic is a professional cooperative partner for the researchers in the field of veterinary diagnostics. We are dedicated to developing animal diseases diagnostic reagents and kits for the laboratory research. For the development of agar gel immunodiffusion kits, we have an R&D team who has have extensive experience and advanced equipments that guarantee high-quality and customized services to our customers. If you have any needs, please don't hesitate to contact us. We will provide you with one-stop feline gastrointestinal pathogens AGID kit development services.
Feline Gastrointestinal Pathogens AGID Kits We Can Develop
| Salmonella radial immunodiffusion enzyme assay kit | Salmonella double immunodiffusion assay kit | Salmonella single immunodiffusion enzyme assay kit |
| Campylobacter jejuni double immunodiffusion assay kit | Campylobacter jejuni radial immunodiffusion enzyme assay kit | Campylobacter jejuni single immunodiffusion assay kit |
| Feline coronaviruses radial immunodiffusion enzyme assay kit | Feline coronaviruses double immunodiffusion assay kit | Feline coronaviruses single immunodiffusion assay kit |
| Other AGID kits you need |
Workflow of Equine Gastrointestinal Pathogens AGID Kit Development
| Workflow | Details |
| Serum sample collection | Blood was collected from sick cats and left to clot at a specific temperature. |
| Gel preparation | Agarose gel was prepared in a specific buffered saline solution at a specific temperature for a given period. |
| Preparation of agar plates | Dispense the melted agar onto a flat surface and prepare the agar plate at a specific temperature. |
| Agar gel immunodiffusion for antibody detection | For each batch of specimens, a strong positive, weak positive, and negative reference serum will be included to aid in interpreting results. |
| Interpretation of test results for antibody detection | Interpret the result via different analysis methods and evaluate the efficiency of the assay. |
Delivery
- A series of AGID kits for feline gastrointestinal pathogens
- Product quality inspection report
- Other experimental data you need
Our Advantages
- High test-to-test repeatability
- Detection of therapeutic pathogens to a level of higher specificity
- Reasonable price and short turnaround time
- Combine AGID with other detection methods to avoid false positives
References
- da Silva, Cristiane Aguero, et al. "Clinical, Ultrasonographic and Histopathological Findings of Gastrointestinal Disorders in Cats." Acta Scientiae Veterinariae 46.1 (2018): 6.
- Levy, Michael G., et al. "Tritrichomonas foetus and not Pentatrichomonas hominis is the etiologic agent of feline trichomonal diarrhea." Journal of Parasitology 89.1 (2003): 99-104.
