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West Nile virus (WNV) was first isolated in 1937 from a woman in the West Nile region of Uganda. It is a small enveloped virus with a diameter of about 50 nm, which belongs to the genus Flavivirus of the family Flaviviridae. The coat of WNV is icosahedral composed of small membrane proteins and an equal amount of E glycoprotein, and its genome is composed of a single-stranded RNA molecule about 11 000 nucleotides long, encoding three structural proteins and seven non-structural proteins. WNV mainly infects birds, humans and mammals such as horses and cattle, causing West Nile fever, which is a seasonal epidemic in North America. The widespread prevalence of the disease has had a huge impact on animal husbandry, animal husbandry and human health.
Figure 1. Organization of West Nile virus particle. A: Representation of West Nile virus particle based on cryoelectron microscopy data. Glycoprotein E is shown on the surface of the particle. Color code: DI (red), DII (yellow) and DIII (blue); B: Ribbon diagram of a dimer of E glycoprotein; C: Structure of a monomer of the soluble ectodomain of E glycoprotein based on the atomic coordinates solved by X-ray crystallography (Martín-Acebes, et al. 2012).
West Nile Virus PCR Kit Development
Prevention and control of West Nile fever requires an integrated approach, including detection of WNV infection and enhanced mosquito control. Virus isolation is the gold standard for WNV detection, but this method is cumbersome and time-consuming, which limits its widespread application. Serological tests and hemagglutination inhibition tests are commonly used laboratory diagnostic methods for WNV. In recent years, with the continuous development of molecular diagnostic technology, various molecular-based diagnostic methods have been applied to the diagnosis of WNV, including traditional RT-PCR, qRT- PCR, multiplex real-time RT-PCR, etc.
BioVenic is a provider of biology reagents and kits, which has been committed to the development of animal pathogens diagnostic reagents for many years. We have a professional team with extensive experience in veterinary diagnostic and reagent development, and our R&D team is working hard to develop and optimize PCR kits. We can provide you with a series of customized PCR kits for the detection of West Nile virus according to your needs. If you have any needs, please feel free to contact us. We will provide you with high-quality products and services.
West Nile Virus PCR Kits We Can Develop
| West Nile virus RT-PCR kit | West Nile virus qRT- PCR kit | West Nile virus multiplex qRT-PCR kit |
| West Nile virus multiplex real-time RT-PCR kit | West Nile virus TaqMan one-step RT- PCR kit | Other West Nile virus PCR kits |
Workflow of West Nile Virus PCR Kit Development
| Workflow | Details |
| Specimen preparation | Specimens can be collected from animals that were infected by West Nile virus. |
| Primer and probes design | Available sequences of West Nile virus can be retrieved from GenBank and specific primer/probe sets were designed. |
| Nucleic acid extraction | Extract RNA from cell cultures, and RNA concentration, purity and integrity were measured. |
| Establishment of PCR conditions | Establishment of thermocycling conditions to conduct amplification and detection through PCR. |
| Reproducibility evaluation of the PCR assay | The reproducibility of the PCR kit was assessed by determining the intra-assay and inter-assay coefficients of variation (CV). |
| Assessment of sensitivity and specificity | The specificity of PCR kit was assessed by detection of RNA from West Nile virus. The sensitivity of the PCR kit was evaluated by determining the limit of detection (LoD). |
Delivery
- A series of PCR kits for West Nile virus
- Product quality inspection report
- Other experimental data you need
Our Advantages
- High reproducibility between tests
- High sensitivity to detect very small amounts of West Nile virus in your samples
- Excellent specificity for the detection of West Nile virus
- Reasonable price and short turnaround time
References
- Martín-Acebes, Miguel A., and Juan-Carlos Saiz. "West Nile virus: a re-emerging pathogen revisited." World Journal of Virology 1.2 (2012): 51.
- Tomar, Priyanka Singh, et al. "Development and evaluation of real-time reverse transcription recombinase polymerase amplification assay for rapid and sensitive detection of West Nile virus in human clinical samples." Frontiers in Cellular and Infection Microbiology 10 (2021): 942.
