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Equine Piroplasmosis is a blood protozoan disease caused by Babesia caballi and Babesia equi parasitizing the red blood cells of equine animals. Clinical symptoms include high fever, anemia, hemorrhage and respiratory distress, and mortality is extremely high if not treated promptly. The disease is transmitted by tick species, so the epidemic is regional and seasonal. Equine piroplasmosis causes serious economic losses to the equine industry. Therefore, it is important to develop an effective detection method for the prevention and control of equine piroplasmosis.
Figure 1. Schematic illustration of the life cycle of Theileria equi (A) and Babesia caballi (B) in the vertebrate host and invertebrate vector (Florin-Christensen, et al.2018).
Equine Piroplasmosis ELISA Kit Development
Diagnosis based on clinical symptoms is challenging because the symptoms of equine piroplasmosis are non-specific. Researchers have developed several serologic tests to diagnose equine piroplasmosis, including the complement binding test (CFT), indirect fluorescent antibody test (IFAT) and enzyme-linked immunosorbent assay (ELISA). These methods facilitate epidemiological studies on large numbers of animals. CFT and IFAT are the most commonly used techniques for detecting equine pear disease. However, CFT does not detect latent infection and does not accurately distinguish between negative and carrier animals, and IFAT is a time-consuming and difficult to standardize method. ELISA is a simple serological method that can be performed on a large number of samples and has been used in recent years as an alternative to CFT and IFAT for the diagnosis of equine piroplasmosis.
BioVenic is a provider of biology reagents & kits, which has been committed to the development of animal pathogen diagnostic reagents for many years. We have a professional team with extensive experience in protein expression and purification and antibody preparation. We can prepare antibodies specific for antigens of Babesia caballi and Babesia equi, and we can provide you with a range of customized ELISA kits for equine piroplasmosis detection according to your needs. If you have any needs, please feel free to contact us, we will provide you with high-quality products.
The ELISA Kits We Can Develop
| Equine piroplasmosis EMA-1 ELISA kit | Equine piroplasmosis EMA-2 ELISA kit | Other equine piroplasmosisELISA kit |
Workflow of ELISA Kit Development
| Workflow | Details |
| Submit ELISA kit development requests | Determine the ELISA development protocol and estimate the cost and cycle based on the assay targets and experimental requirements. |
| Antigen preparation | Design and synthesize primers for the nucleic acid sequence encoding the antigenic protein and obtain the target fragment by amplification, connect the target fragment to the expression vector, induce expression to obtain the recombinant protein, and carry out SDS-PAGE and Western blot to identify its immunogenicity, purification recombinant protein. |
| Antibody preparation | Preparation of antibodies specific for antigens of Babesia caballi and Babesia equi. |
| ELISA kit development | Optimizing the final reaction conditions, evaluation of sensitivity, specificity and concordance of ELISA kit. |
| ELISA kit delivery | We will provide you with customized ELISA kits and product quality inspection report. |
Delivery
A series of ELISA kits for detection equine piroplasmosis
Product quality inspection report (COA)
Other experimental data you need
Our Advantages
- Ready-to-use liquid reagents make assays easy to implement and run
- This kit is highly sensitive and helps in early detection of disease
- Reliable and rapid screening and detection of seropositive animals
- Each kit is rigorously validated and tested
- Reasonable price and short turnaround time
References
- Florin-Christensen, Monica, and Leonhard Schnittger, eds. Parasitic protozoa of farm animals and pets. Springer International Publishing, 2018.
- Asenzo, G., et al. "Development of an indirect ELISA for the diagnosis of equine piroplasmosis." Annals of the New York Academy of Sciences 1149.1 (2008): 235-238.
- Kumar, Sanjay, et al. "Development of EMA-2 recombinant antigen based enzyme-linked immunosorbent assay for seroprevalence studies of Theileria equi infection in Indian equine population." Veterinary Parasitology 198.1-2 (2013): 10-17.
