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Respiratory disease in pigs can be caused by several different pathogens. Enzootic pneumonia (EP) is such an example, characterized by infection with Mycoplasma hyopneumoniae and other bacteria such as Pasteurella multocida, Streptococcus suis, Haemophilus parasuis or Actinobacillus pleuropneumoniae. Some viral pathogens also responsible for porcine respiratory diseases such as porcine respiratory and reproductive syndrome virus (PRRSV), porcine circovirus type 2 (PCV2), swine influenza virus (SIV), etc. The symptoms due to these respiratory pathogens are high fever, diarrhea, lameness, eye redness, paralysis, etc. Every year, the incidence of swine respiratory infections causes huge financial losses to livestock farms.
Figure 1. Schematic diagram of the CSFV life cycle (Li et al., 2017).
Porcine Respiratory Pathogens AGID Kit Development
The agar gel immunodiffusion assay, a diagnostic test that uses serum (the fluid, non-cellular part of blood) to detect antibodies generated in response to infection, is used to effectively treat swine respiratory illnesses caused by several pathogens. For the identification of group-specific antibodies against swine respiratory infections, agar gel immuno-diffusion (AGID) assays have been frequently employed. The agar-gel immunodiffusion test has the benefit of being able to test severely hemolyzed and cytotoxic serum samples with confidence. Various AGID technologies, such as radial immunodiffusion assay (RID), double immunodiffusion assay are widely employed to detect antibodies.
BioVenic has been developing diagnostic reagents and kits for animal diseases for a long time. Our R&D team is hard at work developing and improving agar gel immunodiffusion kits, and we have a capable team with extensive veterinary diagnostic and reagent development experience. We may build a variety of customized agar gel immunodiffusion kits for the identification of porcine respiratory pathogens based on your requirements. Please do not hesitate to contact us, if you have any needs. We will deliver high-quality products and services to you.
Porcine Respiratory Pathogens AGID Kits We Can Develop
| PRRSV double immnodiffusion assay kit | Mycoplasma hyopneumoniae double immnodiffusion assay kit | SIV passive immunodiffusion assay kit |
| PCV2 radial immunodiffusion assay kit | Pasteurella multocida double immnodiffusion assay kit | Other AGID kits you need |
Workflow of Porcine Respiratory Pathogens AGID Kit Development
| Workflow | Details |
| Preparation of medium | Agarose gel was prepared and heated for a given time and then cooled. |
| Preparation of antigen | Antigens were prepared by growing the cells in the prepared medium with the serum. |
| Reference antiserum preparation | Antiserum was prepared by collecting the serum from animals and serum was examined rigorously for specificity. |
| AGID testing | Testing was performed according to the given instructions. |
| Interpretation of results | Tests were read after holding the plates for 24 hours at room temperature and then results were examined and interpreted. |
Delivery
- A set of AGID kits for porcine respiratory pathogens
- Report on product quality
- List of other experimental data you’ll need
Our Advantages
- Reasonable price and short turnaround time
- High reproducibility between tests
- Detects a small number of swine respiratory infections in a high-sensitivity manner
- Detecting therapeutic pathogens with a higher level of specificity
References
- Li, Su, et al. "Complex virus-host interactions involved in the regulation of classical swine fever virus replication: a Minireview." Viruses 9.7 (2017): 171.
- Niederwerder, Megan C. "Role of the microbiome in swine respiratory disease." Veterinary Microbiology 209 (2017): 97-106.
- Chae, Chanhee. "Porcine respiratory disease complex: Interaction of vaccination and porcine circovirus type 2, porcine reproductive and respiratory syndrome virus, and Mycoplasma hyopneumoniae." The Veterinary Journal 212 (2016): 1-6.
