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Influenza virus is a single-stranded RNA virus of the family Orthomyxoviridae, which can be divided into three types: A, B, and C. Influenza A viruses are called avian influenza viruses (AIV) because their natural hosts are birds. Currently, avian influenza A viruses have been isolated from more than 100 different wild birds around the world. The genome of AIV consists of 8 single-stranded RNAs that are packaged as individual rod-shaped ribonucleoprotein complexes (RNPs). Each RNP contains a viral RNA, a viral polymerase and multiple copies of the viral nucleoprotein (NP). Avian influenza is a disease caused by AIV, which is highly contagious and can infect a wide variety of poultry and wild birds. It has a great impact on public health and the poultry industry and caused huge economic losses.
Figure 1. The influenza A virus life cycle (Zheng, et al. 2013).
Avian Influenza Virus ELISA Kit Development
Avian influenza virus can generate new viruses through genetic mutation and combination, so avian influenza has been circulating around the world for many years. The saliva, nasal secretions and feces of AIV-infected birds contain avian influenza virus, and susceptible birds become infected when exposed to these substances. In recent years, with the development of biotechnology, several serological and molecular biological methods have been established to detect avian influenza virus, including reverse transcription loop-mediated isothermal amplification (RT-LAMP), real-time RT-polymerase chain reaction (rRT-PCR), enzyme-linked immunosorbent assay (ELISA), etc. Although these methods are highly sensitive, they are time-consuming and labor-intensive and require specialized equipment. In contrast, ELISA is a simple, cost-effective method for detecting AIV.
BioVenic is a provider of biology reagents & kits, which has been committed to the development of animal virus diagnostic reagents for many years. We have a professional team with extensive experience in protein expression and purification and antibody preparation. We can prepare specific antibodies against the antigens of different avian influenza viruses and can provide you with a series of customized ELISA kits for the detection of avian influenza viruses according to your needs. If you have any needs, please feel free to contact us. We will provide you with high-quality products for animal diagnostics.
The ELISA Kits We Can Develop
| H1N2 ELISA kit | H5N2 ELISA kit | H5N7 ELISA kit |
| H7N1 ELISA kit | H7N7 ELISA kit | H9N9 ELISA kit |
| H10N4 ELISA kit | H16N3 ELISA kit |
Workflow of ELISA Kit Development
| Workflow | Details |
|---|---|
| Submit ELISA kit development requests | Determine the ELISA development protocol and estimate the cost and cycle based on the assay targets and experimental requirements. |
| Antigen preparation | The AIV (H1N2, H5N2, H7N1, H9N9, etc.) antigens were comprehensively analyzed and evaluated to determine the expression intervals of the antigens, and then recombinant proteins with a purity of not less than 90% were prepared for immunization. |
| Antibody preparation | Preparation of antibodies specific for AIV (H1N2, H5N2, H7N1, H9N9, etc.) antigens. |
| ELISA kit development | Antibody label, antibody paired screening, method development and optimization, kit production. |
| ELISA kit delivery | We will provide you with customized ELISA kits and complete product quality inspection report. |
Delivery
- A series of ELISA kits for detection avian influenza viruses
- Product quality inspection report (COA)
- Other experimental data you need
Our Advantages
- High sensitivity and specificity
- High repeatability between tests
- Each kit is rigorously validated and tested
- Professional antibody platform to support ELISA kit development
- Reasonable price and short turnaround time
References
- Chen, Hao, et al. "Development and evaluation of a DAS-ELISA for rapid detection of Tembusu virus using monoclonal antibodies against the envelope protein." PloS One 9.5 (2014): e96366.
- Zheng, W., & Tao, Y. J. (2013). Structure and assembly of the influenza A virus ribonucleoprotein complex. FEBS Letters, 587(8), 1206-1214.
