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Avian reoviruses are members of the Orthoreovirus genus, one of the 12 genera of the Reoviridae family. Avian reovirus is an envelope-free and double-stranded RNA virus. The particle consists of a 60 nm diameter inner protein shell (i.e., core) surrounded by an 81 nm diameter outer protein shell (i.e., coat). Avian reovirus is widespread in poultry. The susceptibility of chickens to avian reoviruses infection is age-dependent, with older chickens being more resistant to infection and virus-induced lesions. Although infection does not usually result in disease, avian reovirus may occasionally be involved in several disease syndromes, of which viral arthritis/tendonitis in chickens is the most important, especially in broiler breeds. Avian reoviruses can cause considerable economic losses in the poultry industry annually.
Figure 1. The avian reovirus replication cycle (Tenorio et al. 2019).
Avian Reovirus PCR Kit Development
Although avian reovirus is ubiquitous among poultry flocks, they have been poorly characterized at the molecular level in the past. However, with the rapid development of biotechnology, various efficient PCR technologies have been developed and used for reoviruses detection. Polymerase chain reaction (PCR), as a diagnostic technique, is generally known as a very sensitive, specific and rapid tool for virus detection. Multiple PCR techniques have been demonstrated for the detection of avian reovirus, including conventional PCR, RT-PCR, real-time RT-PCR, multiplex RT-PCR, duplex rRT-PCR, nested PCR, etc. A variety of optimized PCR techniques are increasingly being used to detect reovirus because they are not only highly sensitive and efficient, but also avoid the high risk that traditional PCR assays may be hampered by contamination of previously amplified material.
BioVenic is a provider of biology reagents and kits, which has been committed to the development of animal virus diagnostic reagents for many years. We have a professional team with extensive experience in veterinary diagnostic and reagent development, and our R&D team is working hard to develop and improve PCR kits. We can provide you with a series of customized PCR kits for the detection of avian reoviruses according to your needs. If you have any needs, please feel free to contact us. We will provide you with high-quality products and services.
Avian Reovirus PCR Kit We Can Develop
| Avian reovirus RT-PCR kit | Avian reovirus real-time PCR kit | Avian reovirus multiplex RT-PCR kit |
| Avian reovirus duplex rRT-PCR kit | Avian reovirus nested PCR kit |
Workflow of Avian Reovirus PCR Kit Development
| Workflow | Details |
| RNA extraction | Puirify the virus and extract RNA from avian reovirus-infected cell cultures. |
| Primer design | A set of primers can be designed according to the sequences of specific strains of avian reovirus using professional software. |
| Establishment of PCR conditions | Maintenance of thermocycling conditions to conduct amplification and detection through PCR. |
| Reproducibility evaluation of the PCR assay | Evaluation of reproducibility of PCR by comparing the avian reovirus-positive samples from different sources. |
| Assessment of sensitivity and specificity | Test negative controls and some avian pathogens including Newcastle disease viruses, infectious bronchitis viruses, infectious bursal disease virus, avian influenza virus, and mycoplasma synovia to evaluate the specificity of the assay. |
Delivery
- A series of PCR kits for avian reovirus
- Product quality inspection report
- Other experimental data you need
Our Advantages
- High sensitivity and specificity
- High reproducibility between tests
- Minimal cross-contamination
- Simple and rapid workflow
References
- Tenorio, Raquel, et al. "Function, architecture, and biogenesis of reovirus replication neoorganelles." Viruses 11.3 (2019): 288.
- Benavente, Javier, and Jose Martínez-Costas. "Avian reovirus: structure and biology." Virus Research 123.2 (2007): 105-119.
- Ke, Guan M., et al. "Development of a quantitative Light Cycler real-time RT-PCR for detection of avian reovirus." Journal of Virological Methods 133.1 (2006): 6-13.
