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Since cattle are ruminants, gastrointestinal tract illness is the most prevalent condition in cow breeding. Hemorrhagic bowel syndrome, winter dysentery, diarrhea, coccidiosis, and other gastrointestinal conditions affect cattle. Bovine coronavirus (BCoV) and bovine viral diarrhea virus (BVDV) are viral pathogens that cause digestive disorders. These gastrointestinal pathogens can cause symptoms like diarrhea, bloating, weight loss, abdominal distention, decreased milk production, etc. Every year, cow farms suffer significant financial losses because of the frequent occurrence of bovine gastrointestinal illnesses.
Figure 1. The morphology and structure of the BVDV virus (Al-Kubati, et al., 2021).
Bovine Gastrointestinal Virus VN Kit Development
The virus neutralization assay, a diagnostic test to identify the existence and quantity of functional systemic antibodies that inhibit a virus's infectivity, is used for the effective management of bovine gastrointestinal diseases brought on by various viral pathogens. The amount of serological cross-reactivity between vaccine antisera and variant BVD viruses, which may be correlated with cross-protection in the host, can be determined using the SVN test. Serological techniques and molecular biological techniques, such as PCR, have been utilized in recent years to diagnose the bovine gastrointestinal virus in the lab. The virus neutralization assay has clear advantages in terms of speed, sensitivity, and specificity when compared to other techniques. Today, a variety of VN approaches, such as the plaque reduction neutralization test and the serum viral neutralization assay are employed to detect antibodies.
BioVenic is a supplier of biological reagents and kits, we devoted a lot of time to developing diagnostic tools and kits for animal diseases. Our assays are developed and processed with higher standards and the results are delivered on time without compromising quality. We have a team with significant expertise in veterinary diagnostic, and we can provide you with a range of specialized VN kits for the detection of gastrointestinal virus infections of bovine. If you have any needs, please feel free to contact us.
Bovine Gastrointestinal Virus VN Kits We Can Provide
| Bocine coronavirus VN kit assay | Coronavirus VN kit assay | BVD virus VN kit |
| BVD virus VN kit assay | Other VN kits you need |
Workflow of Bovine Gastrointestinal Virus VN Kit Development
| Workflow | Details |
| Serum preparation | A two-fold serial dilution of the samples was prepared thrice. |
| Working suspension | Working suspension on standard quantity was prepared. |
| Incubation | Incubation was performed for an hour at a specific range of temperature. |
| Mixing of serum-virus | The serum-virus mixture was added and plated were again considered for incubation. |
| Record of titer | Neutralizing antibody titer was recorded as the reciprocal of the highest serum dilution. |
Delivery
- Inspection report on product quality
- A series of VN kits for bovine gastrointestinal viruses
- Other experimental data you need
Our Advantages
- High sensitivity and specificity
- Viruses can be detected at the strain level
- Detect the level of serological cross reactivity
- Reasonable price and short turnaround time
References
- Peterhans, Ernst, et al., "Cytopathic bovine viral diarrhea viruses (BVDV): emerging pestiviruses doomed to extinction." Veterinary Research 41.6 (2010): 44.
- Jung, Kiwook, et al., "Performance evaluation of three automated quantitative immunoassays and their correlation with a surrogate virus neutralization test in coronavirus disease 19 patients and pre‐pandemic controls." Journal of Clinical Laboratory Analysis 35.9 (2021): e23921.
- Al-Kubati, Anwar AG, et al., "Recent advances on the bovine viral diarrhea virus molecular pathogenesis, immune response, and vaccines development." Frontiers in Veterinary Science 8 (2021): 665128.
