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Respiratory disease is a widespread problem in the sheep flock with presentations varying from acute deaths to chronic ill-thrift. Pathogens across all groups can be represented as causal agents of respiratory disease in sheep. Viruses associated with acute pneumonia include parainfluenza type 3 (PI-3), adenovirus, and respiratory syncytial virus. These viral pneumonia most often affect lambs. Mannheimia haemolytica, Pasteurella multocida, Arcanobacterium pyogenes are some bacterial pathogens that are responsible for respiratory diseases in sheep. M. haemolytica is the most isolated respiratory pathogen according to Veterinary Investigation Diagnosis Analysis (VIDA). Recent investigations suggest that the contribution of pneumonic diseases to lowered sheep productivity has been underestimated. As intensification systems for sheep production are on the increase globally, respiratory diseases have emerged as a more important cause of production loss in the world sheep industry.
Figure 1. Proteomic analysis and immunogenicity of Mannheimia haemolytica(Ayalew et al., 2013).
Sheep Respiratory Pathogens AGID Kit Development
A diagnosis of sheep respiratory disease often requires laboratory confirmation. The agar gel immunodiffusion (AGID) test detects circulating antibodies to group-specific antigens, namely the ribonucleoprotein (RNP) and matrix proteins. The agar gel immunodiffusion kit is compatible with samples from serum and plasma. The immunodiffusion test is most useful for the detection of early primary infection or exacerbation of the existing disease. For the detection of respiratory pathogens (bacterial & viral) in sheep, the single radial immunodiffusion test and Ouchterlony double immunodiffusion test are widely used.
BioVenic is a supplier of biological reagents and kits, and has been developing diagnostic reagents for animal pathogens for many years. Our goal is to be a great company that improves the health and well-being of pets and livestock. Our R&D team is committed to fulfilling all your demands in the development of AGID kits. We can design a variety of customized agar gel immunodiffusion kits for the identification of sheep respiratory pathogens according to your research purposes. If you have any needs, please don't hesitate to contact us. We will provide you with comprehensive sheep respiratory pathogens AGID kit development services.
Sheep Respiratory Pathogens AGID Kits We Can Develop
| Mannheimia haemolytica single radial immunodiffusion assay kit | Pasteurella multocida single radial immunodiffusion assay kit | Arcanobacterium pyogenes single radial immunodiffusion assay kit |
| Respiratory syncytial virus Ouchterlony double immunodiffusion assay kit | Parainfluenza type 3 Ouchterlony double immunodiffusion assay kit | Herpesvirus Ouchterlony double immunodiffusion assay kit |
| Other AGID kits you need |
Workflow of Sheep Respiratory Pathogens AGID Kit Development
| Workflow | Details |
| Serum sample collection | Blood was collected from sick sheep and left to clot at a specific temperature. |
| Gel preparation | Agarose gel was prepared in a specific buffered saline solution at a specific temperature for a given period. |
| Preparation of agar plates | Dispense the melted agar onto a flat surface and prepare the agar plate at a specific temperature. |
| Agar gel immunodiffusion for antibody detection | For each batch of specimens, a strong positive, weak positive, and negative reference serum will be included to aid in interpreting results. |
| Interpretation of test results for antibody detection | Interpret the result via different analysis methods and evaluate the efficiency of the assay. |
Delivery
- A set of AGID kits for sheep respiratory infections
- Product quality control
- List of other experimental data according to your requirements
Our Advantages
- Detecting a small number of sheep respiratory infections in a high-sensitivity manner
- Detecting therapeutic pathogens with a better level of specificity
- Reasonable price and short turnaround time
- Combine AGID with other detection methods to avoid false positives
References
- Ayalew, Sahlu, et al. "Proteomic analysis and immunogenicity of Mannheimia haemolytica vesicles." Clinical and Vaccine Immunology 20.2 (2013): 191-196.
- Wehausen, John D., Scott T. Kelley, and Rob R. Ramey. "Domestic sheep, bighorn sheep, and respiratory disease: a review of the experimental evidence." California Fish and Game 97.1 (2011): 7-24.
