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Streptococcus equi, also known as Streptococcus equi subsp. equi (S. equi), is an equine respiratory symbiotic bacterium that evolved from Streptococcus equi subsp. zooepidemicus (S. zooepidemicus). S. equi belongs to the hemolytic group C streptococci, which can be present in the nasopharynx, tonsils, respiratory tract, and genital mucosa of animals. Strangles is a highly contagious equine upper respiratory disease caused by S. equi and is characterized by fever, mucopurulent nasal discharge, the development of lymph node abscesses, and in severe cases, death by asphyxiation. The widespread spread of Strangles poses a serious threat to the global livestock and horse breeding industries.
Figure 1. Bronchopneumonia by Streptococcus equi subsp. zooepidemicus secondary to inhalation of pine branch of Araucaria angustifolia in a foal, microscopic findings (Molossi, et al. 2021).
Streptococcus equi PCR Kit Development
Strangles is one of the most common equine diseases worldwide and is usually diagnosed by culturing a nasal swab or pus aspirated from an abscess. This method is considered the gold standard for the detection of S. equi. But cell culture takes a long time and requires a lot of technical expertise, and the samples often contain other beta-hemolytic streptococci, which can lead to false negative results. Therefore, there is a need to develop a rapid and highly specific detection method to diagnose S. equi infection and to take corresponding measures in time to control and treat the disease. In recent years, with the continuous development of molecular diagnostic technology, PCR has been widely used in the diagnosis of S. equi infection. Various PCR methods have been developed, including conventional PCR, RT-PCR, real-time PCR,nested PCR, qPCR, etc.
BioVenic is a provider of biology reagents and kits, which has been committed to the development of animal pathogens diagnostic reagents for many years. We have a professional team with extensive experience in veterinary diagnostic and reagent development, and our R&D team is working hard to develop and optimize PCR kits. We can provide you with a series of customized PCR kits for the detection of Streptococcus equi according to your needs. If you have any needs, please feel free to contact us. We will provide you with high-quality products and services.
Streptococcus equi PCR Kit We Can Develop
| S. equi conventional PCR kit | S. equi nested PCR kit | S. equi real-time PCR kit |
| S. equi RT-PCR kit | S. equi qPCR kit | Other S. equi PCR kit |
Workflow of Streptococcus equi PCR Kit Development
| Workflow | Details |
| Primer and probes design | Use oligonucleotide primer analysis software to design primers and probes. |
| Nucleic acid extraction | RNA was extracted from the homogeneous mixture, and RNA concentration, purity and integrity were measured. |
| Establishment of PCR conditions | Establishment of thermocycling conditions to conduct amplification and detection through PCR. |
| Reproducibility evaluation of the PCR assay | The reproducibility of the PCR kit was assessed by determining the intra-assay and inter-assay coefficients of variation (CV). |
| Assessment of sensitivity and specificity | The specificity of PCR kit was assessed by detection of S. equi. The sensitivity of PCR kit was assessed by determine the limit of detection (LoD). |
Delivery
- A series of PCR kits for S. equi
- Product quality inspection report
- Other experimental data you need
Our Advantages
- High sensitivity and high specificity
- High reliability using the internal control
- Professional R&D personnel and technology platform
- Reasonable price and short turnaround time
References
- Molossi, Franciéli Adriane, et al. "Bronchopneumonia by Streptococcus equi subsp. zooepidemicus in a horse with inhalation of pine branch of Araucaria angustifolia." Ciência Rural 52 (2021).
- Webb, Katy, et al. "Detection of Streptococcus equi subspecies equi using a triplex qPCR assay." The Veterinary Journal 195.3 (2013): 300-304.
- North, S. E., et al. "Development of a real‐time PCR to detect Streptococcus equi subspecies equi." Equine Veterinary Journal 46.1 (2014): 56-59.
