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Mycoplasma synoviae is one of the major pathogens causing chronic respiratory disease in poultry worldwide. Avian mycoplasmosis is caused by a variety of pathogenic mycoplasmas, of which Mycoplasma synoviae is one of the most important. M. synoviae infection is usually associated with poor growth, significant carcass downgrading, decreased egg production, and induction of eggshell apex abnormalities. M. synoviae infection usually occurs as a subclinical upper respiratory disease, but when co-infected with other viral or bacterial pathogens, it can lead to severe air-sensitive arthritis or synovitis. M. synoviae can be transmitted both vertically in breeders and horizontally, particularly in layers in multi-age farms. Although M. synoviae does not directly cause poultry mortality, it still causes serious economic losses to chicken and turkey production every year.
Figure 1. Transmission ways of Mycoplasma synoviae in poultry (Kaboudi et al. 2019).
Mycoplasma synoviae PCR Kit Development
M. synoviae infections caused by the poor biosecurity of large poultry populations. the only effective way of control is to maintain M. synoviae–free breeder flocks. Traditional M. synoviae monitoring protocols rely on serologic testing to screen for or verify M. synoviae infection. However, recent studies have revealed limitations of serologic testing, such as delayed serologic response in chickens. With the rapid development of biotechnology, various PCR techniques have been established for the detection of M. synoviae. Among them, some improved PCR techniques such as multiplex PCR, real-time PCR, duplex real-time TaqMan PCR and multiplex RT-PCR are frequently used for a rapid detection of M. synoviae because of their excellent specificity, high efficiency and high sensitivity.
BioVenic is a provider of biology reagents and kits, which has been committed to the development of animal virus diagnostic reagents for many years. We have a professional team with extensive experience in veterinary diagnostic and reagent development, and our R&D team is working hard to develop and improve PCR kits. We can provide you with a series of customized PCR kits for the detection of Mycoplasma synoviae according to your needs. If you have any needs, please feel free to contact us. We will provide you with high-quality products and services.
Mycoplasma synoviae PCR Kit We Can Develop
| M. synoviae multiplex PCR kit | M. synoviae real-time PCR kit |
| M. synoviaeduplex real-time TaqMan PCR kit | M. synoviae multiplex RT-PCR kit |
Workflow of Mycoplasma synoviae PCR Kit Development
| Workflow | Details |
| Sample preparation and DNA extraction | Specimen for M. synoviae isolation can be taken from M. synoviae-positive live birds, then extract DNA from the sample for the PCR reaction. |
| Primers and probes designing | Selection of specific primers and probes for the detection of M. synoviae. |
| Establishment of PCR conditions | Maintenance of thermocycling conditions to conduct amplification and detection through PCR. |
| Reproducibility evaluation of the PCR assay | Evaluation of reproducibility of PCR by comparing the M. synoviae-positive samples from different sources. |
| Sentivity analysis | Evaluation of PCR sensitivity by using 10-fold serial dilution of extracted DNA of M. synoviae strain. |
Delivery
- A series of PCR kits for Mycoplasma synoviae
- Product quality inspection report
- Other experimental data you need
Our Advantages
- A highly sensitive and specific PCR test kit
- High repeatability between tests
- Ensures the early and reliable detection
- Reasonable price and short turnaround time
- Simple and rapid workflow
References
- Chaidez‐Ibarra, Miguel Angel, et al. "Pooled molecular occurrence of Mycoplasma gallisepticum and Mycoplasma synoviae in poultry: A systematic review and meta‐analysis." Transboundary and Emerging Diseases (2021).
- Kaboudi, Khaled, and Adem Jbenyeni. " Mycoplasma synoviae infection in layers: diagnosis and control measures–a review." Archives of Veterinary Medicine 12.2 (2019): 63-82..
- Tebyanian, Hamid, et al. "Isolation and identification of Mycoplasma synoviae from suspected ostriches by polymerase chain reaction, in kerman province, iran." Jundishapur Journal of Microbiology 7.9 (2014).
