Services
Mycobacterium tuberculosis complex (MTBC) consists of a variety of highly genetically related strains, broadly divided into those that infect humans and those that infect various wild and domesticated animals. Since 1896, MTBC that have been identified to infect animals include: M. mungi, M. caprae, M. microti, Dassie bacillus, M. pinnipedii, M. orygis and M. suricattae. These pathogens have a wide range of animal hosts including: Including: dogs, cats, cows, birds, voles, deer, pigs, goats, mice, badgers, seals and rhinos. Tuberculosis (TB) is an infectious disease caused by the Mycobacterium tuberculosis complex (MTBC) that has significant implications for human and animal health. In addition, it has important economic implications for wild and domestic animals worldwide. Therefore, it is crucial to develop a rapid and accurate method for diagnosing MTBC.
Figure 1. The molecular markers and regions of deletion that distinguish the animal adapted species of the Mycobacterium tuberculosis complex (Malone, et al. 2017).
Mycobacterium tuberculosis Complex PCR Kit Development
Early detection of MTBC-infected animals is critical to reduce the incidence of TB. Currently, intradermal tuberculin test, bacterial culture and gamma interferon release test have been used for the diagnosis of MTBC. However, these methods are relatively insensitive, expensive and labor-intensive. Compared with conventional methods, PCR is one of the most promising and attractive diagnostic tools for MTBC due to its advantages of rapidity, sensitivity and accuracy. Researchers have developed a variety of PCR-based MTBC detection methods, including conventional PCR, real-time PCR,nested PCR, multiplex PCR, etc.
BioVenic is a provider of biology reagents and kits, which has been committed to the development of animal pathogens diagnostic reagents for many years. We have a professional team with extensive experience in veterinary diagnostic and reagent development, and our R&D team is working hard to develop and optimize PCR kits. We can provide you with a series of customized PCR kits for the detection of Mycobacterium tuberculosis complex according to your needs. If you have any needs, please feel free to contact us. We will provide you with high-quality products and services.
Mycobacterium tuberculosis Complex PCR Kit We Can Develop
| MTBC conventional PCR kit | MTBC nested PCR kit | MTBC real-time PCR kit |
| MTBC RT-PCR kit | MTBC multiplex PCR kit | Other MTBC PCR kit |
Workflow of Mycobacterium tuberculosis ComplexPCR Kit Development
| Workflow | Details |
| MTBC microbiological culture | Fresh bovine retropharyngeal, tracheobronchial and mesenteric lymph node tissue samples were collected, ground to a homogeneous mixture, and then subjected to selective bacterial culture. |
| Primer and probes design | Use oligonucleotide primer analysis software to design primers and probes targeting the mpb70 gene, specific for MTBC members. |
| Nucleic acid extraction | DNA/RNA was extracted from the homogeneous mixture, and DNA/RNA concentration, purity and integrity were measured. |
| Establishment of PCR conditions | Establishment of thermocycling conditions to conduct amplification and detection through PCR. |
| Reproducibility evaluation of the PCR assay | The reproducibility of the PCR kit was assessed by determining the intra-assay and inter-assay coefficients of variation (CV). |
| Assessment of sensitivity and specificity | The specificity of PCR kit was assessed by detection of seven species of the M. tuberculosis complex. The sensitivity of PCR kit was assessed by determine the limit of detection (LoD) at the DNA levels. |
Delivery
- A series of PCR kits for MTBC
- Product quality inspection report
- Other experimental data you need
Our Advantages
- High sensitivity and high specificity
- High reliability using the internal control
- Detects all Mycobacteria belonging to the Mycobacterium tuberculosis complex
- Professional R&D personnel and technology platform
- Reasonable price and short turnaround time
References
- Malone, Kerri M., and Stephen V. Gordon. "Mycobacterium tuberculosis complex members adapted to wild and domestic animals." Strain Variation in the Mycobacterium tuberculosis Complex: Its Role in Biology, Epidemiology and Control (2017): 135-154.
- Lorente-Leal, Victor, et al. "Validation of a real-time PCR for the detection of Mycobacterium tuberculosis complex members in bovine tissue samples." Frontiers in Veterinary Science 6 (2019): 61.
