Bovine respiratory infection refers to all disorders of the respiratory system in cattle that are mostly viral in origin. The common viral pathogens of bovine respiratory infections include bovine viral diarrhea virus, bovine infectious rhinotracheitis virus, bovine respiratory syncytial virus, bovine parainfluenza virus 3, bovine adenovirus 3, bovine adenovirus 7, bovine coronavirus, etc. These viruses have the potential to harm the respiratory system's natural defensive mechanisms in cattle. The normal nasopharyngeal host bacteria will then descend and lodge in the host's lungs, presenting various respiratory symptoms. At the same time, the overwhelming host reaction to viral infection will further destroy the respiratory tract's defense capability. Bovine respiratory viruses are a serious threat to the global dairy and beef industries and have a large negative financial impact on the cattle industry. As a result, early pathogen diagnosis is crucial for the prompt management and control of bovine respiratory infections.
Figure 1. Electron micrograph of bovine adenovirus-3 (Gaba, et al., 2019).
The interaction of several pathogens with cattle results in bovine respiratory diseases, and the clinical symptoms of infection by multiple pathogens are similar. As a result, techniques like pathogen isolation and clinical signs cannot reliably identify pathogens. It is essential to create a precise, quick, and thorough approach for the identification of the bovine respiratory virus to effectively control the development of BRDC and limit economic losses. Serological techniques and molecular biological techniques, such as PCR, have been utilized in recent years to diagnose the bovine respiratory virus in the lab. The virus neutralization assay has clear advantages in terms of speed, sensitivity, and specificity when compared to other techniques.
BioVenic is a supplier of biological reagents and kits, and has been developing diagnostic reagents for animal viruses for many years. Our goal is to be a great company that improves the health and well-being of pets and livestock. We have a professional team with extensive experience in veterinary diagnostics and reagent development, and we can provide you with a series of customized virus neutralization kits for the detection of bovine respiratory virus based on your needs. If you have any queries, please feel free to contact us. We will provide you with high-quality products and services.
Bovine diarrhea virus VN kit assay | Bovine respiratory syncytial virus VN kit assay | Bovine infectious rhinotracheitis virus VN kit assay |
Bovine parainfluenza virus 3 VN kit assay | Other VN kits you need |
Workflow | Details |
Viral source | Live virus strains and characterized bovine parainfluenza virus strains were obtained. |
Sample collection | Blood samples were obtained from industrial breeding firms. |
VN assay optimization | For the optimal neutralization of homologous antigen and antibody under controlled conditions, variables were tuned during the investigation. |
Centrifugation | A suspension of the antigen and the antiserum was centrifuged for five minutes. The air sac aperture was used to inject supernatant. |
Control group | Serum samples were obtained and using homologous antigens, the samples were examined for relevant viral exposure. |
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