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Equine infectious anemia virus (EIAV) is a member of the genus Lentivirus in the family Retroviridae. EIAV is a blood-borne virus that is transmitted from one animal to another by blood-feeding insects or contaminated needles. EIAV mainly infects equine animals causing equine infectious anemia (EIA), which manifests as fever, anemia, heart weakness, weight loss and other symptoms. Infected horses are lifelong carriers of EIAV. EIAV has a rapid transmission rate and high mortality rate seriously endangers the health of equine animals and causes serious economic losses to the horse breeding industry.
Figure 1. Cartoon of the equine infectious anaemia virion structure showing the location and identity of structural proteins (Issel, et al. 2015).
Equine Infectious Anemia Virus ELISA Kit Development
Currently, the main measure to prevent EIA infection is to test horses for EIAV and culling them if positive. Therefore, it is necessary to develop simple and effective methods to detect EIAV. The most reliable indicator of EIAV infection is the detection of its specific antibodies in horse serum. Serological methods are commonly used for EIAV detection in worldwide, including agar gel immunodiffusion (AGID), enzyme-linked immunosorbent assay (ELISA), etc. Although AGID is inexpensive and specific, the test takes a considerable amount of time and is less sensitive. ELISA is sensitive, specific and easy to program, so it is widely used for the detection of EIAV.
BioVenic is a provider of biology reagents & kits, which has been committed to the development of animal virus diagnostic reagents for many years. We have a professional team with extensive experience in protein expression and purification and antibody preparation. We can prepare monoclonal antibodies specific for individual antigens of equine infectious anemia virus or antibodies against whole virus multi-antigens, so we can provide you with a range of customized ELISA kits for equine infectious anemia virus detection according to your needs. If you have any needs, please feel free to contact us. We will provide you with high-quality products for animal diagnostics.
The ELISA Kits We Can Develop
| EIAV gp45-ELISA kit | EIAV gp90-ELISA kit |
| EIAV g26-ELISA kit | EIAV whole-virus ELISA kit |
Workflow of ELISA Kit Development
| Workflow | Details |
|---|---|
| Submit ELISA kit development requests | Determine the ELISA development protocol and estimate the cost and cycle based on the assay targets and experimental requirements |
| Antigen preparation | DNA extraction, cloning and sequencing of genes encoding the proteins gp45, gp90 and g26, and then constructing vectors to express recombinant proteins. |
| Antibody preparation | Preparation of monoclonal antibodies specific for individual antigens of EIAV or antibodies against whole virus multi-antigens. |
| ELISA kit development | Antibody label, antibody paired screening, method development and optimization, kit production, kit evaluation. |
| ELISA kit delivery | We will provide you with customized ELISA kits and complete product quality inspection report. |
Delivery
- A series of ELISA kits for detection equine infectious anemia virus
- Product quality inspection report (COA)
- Other experimental data you need
Our Advantages
- High sensitivity and specificity
- Less equipment and greater flexibility
- Each kit is rigorously validated and tested
- Positive results are read directly and visually
- Reasonable price and short turnaround time
References
- Alvarez, Irene, et al. "The efficacy of ELISA commercial kits for the screening of equine infectious anemia virus infection." Revista Argentina de Microbiología 47.1 (2015): 25-28.
- Issel, C. J., and L. D. Foil. "Equine infectious anaemia and mechanical transmission: man and the wee beasties." Rev. Sci. Tech. OIE 34 (2015): 513-523.
- Craigo, Jodi K., Corin Ezzelarab, and Ronald C. Montelaro. "Development of a high throughput, semi-automated, infectious center cell-based ELISA for equine infectious anemia virus." Journal of Virological Methods 185.2 (2012): 221-227.
