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Toxoplasma gondii (T. gondii) was discovered by French scholars in 1980 in the spleen mononuclear cells of the gopher rat. It belongs to the genus Toxoplasma in the family Sarcocystidae. T. gondii is an intracellular parasitic protozoan, and the feline is the final host. T. gondii reproduces sexually in the small intestinal epithelial cells of felines, and its oocysts are excreted with the shed small intestinal epithelial cells. Animals other than felines are only intermediate hosts and do not have oocysts in the feces after infection with T. gondii, but do have cysts in body tissues. The main route of transmission of T. gondii is ingestion of undercooked meat containing cysts or food contaminated with feces containing oocysts. It poses a great threat to the health of cats around the world and causes huge economic losses. Therefore, the diagnosis of T. gondii is crucial for the monitoring, prevention and control of toxoplasmosis.
Figure 1. Life cycle of T. gondii (From Wikipedia).
Toxoplasma gondii PCR Kit Development
Currently, the diagnosis of toxoplasmosis mainly relies on serological testing and cell culture. While serological testing allows for rapid and large-scale screening for toxoplasmosis, it cannot detect specificity antibodies in the early stages of toxoplasmosis because these antibodies are produced several weeks after infection. Cell culture is time-consuming and labor-intensive, and is not suitable for rapid diagnosis of T. gondii. In recent years, PCR technology has been increasingly used to diagnose toxoplasmosis because it is a rapid, sensitive, and accurate diagnostic tool. Several PCR-based techniques have been developed for the diagnosis of toxoplasmosis, including nested PCR, real-time PCR, PCR-RFLP, triplex PCR, etc.
BioVenic is a provider of biology reagents and kits, which has been committed to the development of animal pathogens diagnostic reagents for many years. We have a professional team with extensive experience in veterinary diagnostic and reagent development, and our R&D team is working hard to develop and optimize PCR kits. We can provide you with a series of customized PCR kits for the detection of Toxoplasma gondii according to your needs. If you have any needs, please feel free to contact us. We will provide you with high-quality products and services.
Toxoplasma gondii PCR Kit We Can Develop
| T. gondii conventional PCR kit | T. gondii nested PCR kit | T. gondii real-time PCR kit |
| T. gondii PCR-RFLP kit | T. gondii triplex PCR kit | Other T. gondii PCR kit |
Workflow of Toxoplasma gondii PCR Kit Development
| Workflow | Details |
| T. gondii strain cultures | T. gondii strain from different samples were cultured and isolation. |
| Primer and probes design | Design primers or probes to detect T. gondii, and use professional software to select and evaluate primers and probes. |
| Nucleic acid extraction | DNA was extracted from tachyzoites of the T. gondii strain, and DNA concentration, purity and integrity were measured. |
| Establishment of PCR conditions | Establishment of thermocycling conditions to conduct amplification and detection through PCR. |
| Reproducibility evaluation of the PCR assay | The reproducibility of the PCR kit was assessed by determining the intra-assay and inter-assay coefficients of variation (CV). |
| Assessment of sensitivity and specificity | The specificity of PCR kit was assessed by detection of DNA from T. gondii and other pathogens. The sensitivity of PCR kit was assessed by determine the limit of detection (LoD) at the DNA and parasite levels. |
Delivery
- A series of PCR kits for T. gondii
- Product quality inspection report
- Other experimental data you need
Our Advantages
- High performance and high specificity
- High repeatability between tests
- Ready-to-use master mix
- Professional R&D personnel and technology platform
- Reasonable price and short turnaround time
References
- Marino, Anna Maria Fausta, et al. "Development and validation of a real-time PCR assay for the detection of Toxoplasma gondii DNA in animal and meat samples." Journal of Veterinary Diagnostic Investigation 29.2 (2017): 203-207.
- Rahumatullah, Anizah, Boon Yin Khoo, and Rahmah Noordin. "Triplex PCR using new primers for the detection of Toxoplasma gondii." Experimental Parasitology 131.2 (2012): 231-238.
