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Canine parvovirus (CPV) is a non-enveloped, single-stranded DNA virus of the genus Proparovirus in the Parvoviridae family. Canine parvovirus disease is an acute, contact and highly lethal infectious disease caused by CPV infection, and it is one of the most common infectious diseases in dogs worldwide. In 1978, Eugster first discovered CPV-2 in the feces of a dog suffering from hemorrhagic enteritis in American. Subsequently, the virus spread globally, and three antigenic variants CPV-2a, CPV-2b and CPV-2c soon emerged. CPV is transmitted by the fecal-oral route. When dogs are infected with any type of CPV, they develop similar symptoms such as vomiting, diarrhea, loss of appetite, and dehydration. the widespread spread of CPV has caused significant economic losses to the dog breeding industry. Therefore, it is necessary to develop a rapid and accurate method for detecting CPV.
Figure 1. Transmission and clinical symptoms of canine parvovirus type 2 (CPV‐2) (Lin, et al. 2016).
Canine Parvovirus PCR Kit Development
CPV infection leads to high morbidity and mortality, and dogs can die within days if left untreated. To detect CPV infection as early as possible, researchers have developed a variety of laboratory tests, including electron microscopy (EM), virus isolation, fluorescent antibody test (FAT), enzyme-linked immunosorbent assay (ELISA), CIE test, latex agglutination Test (LAT), PCR, etc. Recently, PCR technology has been increasingly used to diagnose canine parvovirus infection because it is a rapid, sensitive, and accurate diagnostic tool compared to other methods. Several PCR assays have been reported, such as conventional PCR, nested PCR, iiPCR, SYBR Green real-time PCR.
BioVenic is a provider of biology reagents and kits, which has been committed to the development of animal pathogens diagnostic reagents for many years. We have a professional team with extensive experience in veterinary diagnostic and reagent development, and our R&D team is working hard to develop and optimize PCR kits. We can provide you with a series of customized PCR kits for the detection of canine parvovirus according to your needs. If you have any needs, please feel free to contact us. We will provide you with high-quality products and services.
Canine Parvovirus PCR Kit We Can Develop
| CPV-2 conventional PCR kit | CPV-2 nested PCR kit | CPV-2 TaqMan RT-PCR kit |
| CPV-2 real-time PCR kit | CPV-2 RT-PCR kit | CPV-2a real-time PCR kit |
| CPV-2a conventional PCR kit | CPV-2a nested PCR kit | CPV-2a TaqMan RT-PCR kit |
| CPV-2a RT-PCR kit | CPV-2b conventional PCR kit | CPV-2b nested PCR kit |
| CPV-2b real-time PCR kit | CPV-2b RT-PCR kit | CPV-2b real-time PCR kit |
| CPV-2c conventional PCR kit | CPV-2c nested PCR kit | CPV-2c TaqMan RT-PCR kit |
| CPV-2c real-time PCR kit | CPV-2c RT-PCR kit | Other CPV PCR kit |
Workflow of Canine Parvovirus PCR Kit Development
| Workflow | Details |
| Virus and cell cultures | Propagation and isolation of CPVs from different samples. |
| Primer and probes design | Design primers or probes to detect CPV-2, CPV-2a, CPV-2b and CPV-2c, and use professional software to select and evaluate primers and probes. |
| Nucleic acid extraction | Extract DNA from cell culture supernatants or fecal samples of infected dogs. |
| Establishment of PCR conditions | Establishment of thermocycling conditions to conduct amplification and detection through PCR. |
| Reproducibility evaluation of the PCR assay | The reproducibility of the PCR kit was assessed by determining the intra-assay and inter-assay coefficients of variation (CV). |
| Assessment of sensitivity and specificity | The specificity of PCR kit was assessed by detection of CPV-2, CPV-2a, CPV-2b and CPV-2c as well as various other unrelated canine virus strains (CPV-2c). The sensitivity of PCR kit was assessed by detection serial 10-fold dilutions of each standard DNA plasmid. |
Delivery
- A series of PCR kits for canine parvovirus
- Product quality inspection report
- Other experimental data you need
Our Advantages
- High purity Taq DNA Polymerase
- Highly specific detection profile
- Ready-to-use master mix
- Professional R&D personnel and technology platform
- Reasonable price and short turnaround time
References
- Lin, Chao-Nan, and Shu-Yun Chiang. "Canine parvovirus type 2." Canine Medicine–Recent Topics and Advanced Research. Kaoud HAE (Ed.). In Tech Publ., Rijeka, Croatia (2016): 1-17.
- Sun, Yaru, et al. "A multiplex TaqMan real-time PCR for detection and differentiation of four antigenic types of canine parvovirus in China." Molecular and Cellular Probes 38 (2018): 7-12.
- Elia, Gabriella, et al. "Detection of infectious canine parvovirus type 2 by mRNA real-time RT-PCR." Journal of Virological Methods 146.1-2 (2007): 202-208.
