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There are a number of diseases that dogs are susceptible to that can be easily treated. Most of these diseases are not serious, however, if they go undetected and therefore untreated, some do have the potential to pose a serious health issue to the dogs. Most common diseases include canine distemper, canine hepatitis, canine influenza, canine parainfluenza, kennel cough, etc. The pathogens that lead to these diseases can be bacteria (Coxiella burnetii, Bordetella bronchiseptica, Leptospira interrogans and Staphylococcus intermedius), viruses (Rabies, Noroviruses, and canine influenza viruses), and parasites (Toxocara cati, Toxascaris leonina, Trichuris vulpis, Dipylidium caninum, etc). Infection prevention and control measures can be broadly divided into two main categories: measures to decrease dog exposure to infectious agents and measures to decrease dog vulnerability to infectious agents. Identification of the canine pathogens is essential to understand the canine diseases and constitute the prevention and control strategy of canine infectious diseases.
Figure 1. R. sanguineus (s.l.) infested dogs' blood parasite examination results (Bartolome-Cruz, et al., 2018).
Canine Pathogens IFA Kit Development
In order to addressed treatment, prevention, prognosis, and zoonotic issues optimally, it's important to gather information of cainine pathogens via diagnostic test results. Immunofluorescence assay (IFA) is a frequently used method for the dectection of canine pathogens, It's based on the principle of antigen-antibody reaction. First, the known antigen or antibody is labeled with fluorescein, then the fluorescent antibody (or antigen) is used as a molecular probe to check the corresponding antigen (or antibody) in cells or tissues. When observing the specimen with a fluorescence microscope, fluorescein emits bright fluorescence under the irradiation of stimulated luminescence, and the cells or tissues where the fluorescence is located can be spotted, so as to determine the nature and location of the antigen or antibody. Direct IFA and indirect IFA have been developed by researchers for the identification of canine pathogens.
BioVenic is a supplier of biological reagents and kits, and has been developing diagnostic reagents for animal pathogens for many years. Our goal is to be a great company that improves the health and well-being of pets and livestock. We have a professional team with extensive experience in veterinary diagnostic and kit development, and we can develop a series of customized immunofluorescence kits for the identification of canine pathogens based on your requirements. Please do not hesitate to contact us, we will provide high-quality products and services.
Canine Pathogens IFA Kits We Can Develop
| Coxiella burnetii direct IFA kit | Coxiella burnetii indirect IFA kit | Canine influenza virus direct IFA kit |
| Canine influenza virus indirect IFA kit | Rabies direct IFA kit | Rabies indirect IFA kit |
| Toxocara cati direct IFA kit | Toxocara cati indirect IFA kit | Other IFA kits you need |
Workflow of Canine Pathogens IFA Kit Development
| Workflow | Details |
| Sample preparation | Tissue sample or blood sample was taken from the sick dogs. |
| Sample fixation | Incubating the sample for 10 minutes at room temperature in a formalin solution or chilled methanol or acetone to crosslink the proteins. |
| Cell permeabilization | To stain intracellular proteins, permeabilize the cell by incubating in a detergent. |
| Blocking | To minimize intra- or extracellular background signals, block the non-specific antigens by incubating the sample in the serum of the host, in which the secondary antibody was made. |
| Antibody incubation | Primary antibody and secondary antibody were incubated in selected solutions successively. |
| Microscopy | Process the microscopic analysis after counterstain and mounting. |
| Assessment of the assay | Evaluate the assay by digital image analysis and statistical analysis. |
Delivery
- A series of IFA kits for canine pathogens
- Product quality inspection report
- Other experimental data you need
Our Advantages
- High reproducibility between tests
- High specificity and less nonspecific fluorescent staining
- Only minute amounts of sample and antibody conjugate is required
- The multiple targets can be detected in the single sample
- Reasonable price and short turnaround time
References
- Bartolome-Cruz, Kathlyn. "Detection of pathogens on the brown dog tick, Rhipicephalus sanguineus sensu lato (sl)(Arachnida: Acari: Ixodidae) in the Philippines." Philippine Journal of Science 147.4 (2018): 741-751.
- Llera, JL Guillén, et al., "Differential serological testing by simultaneous indirect immunofluorescent antibody test in canine leishmaniosis and ehrlichiosis." Veterinary Parasitology 109.3-4 (2002): 185-190.
