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Bordetella avium (B. avium) is an aerobic or facultative anaerobic fermentative Gram-negative bacillus belonging to the genus Bordetella. B. avium infection causes a highly contagious avian upper respiratory disease that is widespread in wild and domesticated birds in many countries, causing significant economic losses to the poultry industry. Commercial turkeys being the largest host of B. avium, and the incidence of B. avium infection in turkeys is high, but the mortality rate is less than 10%.
Figure 1. SEM of tracheae incubated with B. avium (Temple, et al.1998).
Bordetella avium ELISA Kit Development
Currently, a variety of methods have been developed for the identification of B. aviums, including agglutination tests, fluorescent antibody techniques, isolation, agar gel precipitin tests, and indirect ELISA. Each method has its own advantages and disadvantages, such as time-consuming steps, low sensitivity, complex operation and poor antibody specificity. Monoclonal antibodies can specifically recognize antigens on the surface of pathogens and have a high sensitivity, which has an irreplaceable role in the detection of pathogenic microorganisms. Therefore, ELISA kits based on monoclonal antibodies are widely used in the diagnosis and prevention of B. avium infections.
BioVenic is a provider of biology reagents & kits, which has been committed to the development of animal pathogen diagnostic reagents for many years. We have a professional team with extensive experience in protein expression and purification and antibody preparation. We can prepare monoclonal antibodies specific for B. avium antigens or antibodies against B. avium multi-antigens, so we can provide you with a range of customized ELISA kits for B. avium detection according to your needs. In addition, we can also provide you with ELISA kits to detect cytokine changes in avians inoculated with vaccines. If you have any needs, please feel free to contact us. We will provide you with high-quality products for animal diagnostics.
The ELISA Kits We Can Develop
| B. avium IgG-ELISA kit | B. avium IFN-γ-ELISA kit |
| B. avium DAS-ELISA kit | B. avium IL-2-ELISA kit |
| B. avium IL-4-ELISA kit | Other B. avium ELISA kit |
Workflow of ELISA Kit Development
| Workflow | Details |
| Submit ELISA kit development requests | Determine the ELISA development protocol and estimate the cost and cycle based on the assay targets and experimental requirements. |
| Antigen preparation | Preparation of secreted proteins, extraction of secreted antigens, preparation of soluble antigens of B. avium. |
| Antibody preparation | Preparation of monoclonal antibodies specific for B. avium antigens or antibodies against B. avium multi-antigens, analysis for antibodies specificity and epitope. |
| ELISA kit development | Optimizing the best concentration of B. avium antigens and antibody dilution, optimizing the sensitivity and specificity of ELISA kit. |
| ELISA kit delivery | We will provide you with customized ELISA kits and product quality inspection report. |
Delivery
A series of ELISA kits for detection B. avium
Product quality inspection report (COA)
Other experimental data you need
Our Advantages
- Highly sensitive and specific
- Use for routine B. avium flock profiling and B. avium vaccination evaluation
- Each kit is rigorously validated and tested
- Enables differentiation of weak from strong positive samples
- Reasonable price and short turnaround time
References
- Liu, Guanhua, et al. "Monoclonal antibodies directed against the outer membrane protein of Bordetella avium." Monoclonal Antibodies in Immunodiagnosis and Immunotherapy 32.4 (2013): 295-300.
- Temple, Louise M., et al. "Bordetella avium virulence measured in vivo and in vitro." Infection and Immunity 66.11 (1998): 5244-5251.
