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Mycobacterium avium subspecies paratuberculosis (MAP) is an obligate pathogenic bacterium in the genus Mycobacterium. It is often abbreviated M. paratuberculosis or M. avium ssp. paratuberculosis. Johne's disease (JD) is a chronic wasting disease caused by MAP that is widely prevalent in domestic animals, wild ruminants and other animal species and can lead to weight loss, wasting and diarrhea. MAP infection reduces the quality and quantity of dairy and meat products, causing significant economic losses to the livestock farming, animal husbandry and dairy industries. Therefore, finding rapid and accurate methods to diagnose MAP infection in animals is important for the control and treatment of JD and reduce economic losses in livestock farming, dairy and animal husbandry.
Figure 1. Mycobacterium avium subsp. paratuberculosis (MAP) properties. (A) Acid-fast stain of intestinal epithelium from an experimentally challenged bovine reveals MAP (red rods) inside macrophages. (B) Electron microscopy clearly shows the rod-shaped mycobacteria magnified over 50,000 times (Rathnaiah, et al.2017).
M. paratuberculosis ELISA Kit Development
The control and eradication of MAP is challenging due to its long latency period, insidious nature and the lack of rapid and accurate detection methods. Currently, researchers have developed several MAP detection methods, such as fecal culture, ELISA and PCR. Although fecal culture is considered the gold standard for identifying MAP, it has the shortcomings of being time-consuming, labor-intensive, with limited sensitivity and expensive. PCR is a method that detection MAP infection by detecting DNA of MAP in feces, but the low amount of MAP in feces makes it challenging to isolate MAP DNA. In addition, the presence of PCR inhibitors in feces can affect the sensitivity of PCR for MAP detection. Compared to fecal culture and PCR, ELISA is simple and easy to operate, has high specificity and can detect a large number of serum samples, and is a common method to detect MAP infection.
BioVenic is a provider of biology reagents & kits, which has been committed to the development of animal pathogen diagnostic reagents for many years. We have a professional team with extensive experience in protein expression and purification and antibody preparation. We can prepare antibodies specific for MAP cell envelope proteins, and we can provide you with a range of customized ELISA kits for MAP detection according to your needs. If you have any needs, please feel free to contact us. We will provide you with high-quality products for diagnosis of animal diseases.
The ELISA Kits We Can Develop
| MAP SdhA ELISA kit | MAP FadE25-2 ELISA kit | MAP FadE3-2 ELISA kit |
| MAP Mk1 ELISA kit | MAP DesA2 ELISA kit | MAP MAP1233 ELISA kit |
| MAP total proteins ELISA kit | Other MAPELISA kit |
Workflow of ELISA Kit Development
| Workflow | Details |
| Submit ELISA kit development requests | Determine the ELISA development protocol and estimate the cost and cycle based on the assay targets and experimental requirements. |
| Antigen preparation | Bacteria strains and culture, DNA extraction, polymerase chain reaction and gene cloning, expression and purification of MAP cell envelope proteins. |
| Antibody preparation | Preparation of antibodies specific for total cell envelope proteins or individual protein of MAP, analysis for antibodies specificity and epitope. |
| ELISA kit development | Optimizing the best concentration of MAP cell envelope proteins and antibody dilution, evaluation of sensitivity, specificity and concordance of ELISA kit. |
| ELISA kit delivery | We will provide you with customized ELISA kits and product quality inspection report. |
Delivery
A series of ELISA kits for detection MAP
Product quality inspection report (COA)
Other experimental data you need
Our Advantages
- Highly specificity, low background
- Minimal cross-contamination
- Each kit is rigorously validated and tested
- Reasonable price and short turnaround time
References
- Malamo, Mumeka, et al. "Development of ELISA to detect antibodies specific to Mycobacterium avium subsp. paratuberculosis with truncated34kDa proteins." Japanese Journal of Veterinary Research 54.2-3 (2006): 99-107.
- Rathnaiah, Govardhan, et al. "Pathogenesis, molecular genetics, and genomics of Mycobacterium avium subsp. paratuberculosis, the etiologic agent of Johne's disease." Frontiers in Veterinary Science 4 (2017): 187.
- Karuppusamy, Shanmugasundaram, et al. "Detection of Mycobacterium avium subspecies paratuberculosis (MAP) microorganisms using antigenic MAP cell envelope proteins." Frontiers in Veterinary Science 8 (2021): 52.
