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Multiple viral infections and multiple factors seem to contribute to canine respiratory diseases. One or more bacterial and viral species can be the cause of the highly common canine infectious respiratory disease complex (CIRDC). Cough, drainage from the eyes or nose, fever, and other mild or severe symptoms of the disease include pneumonia exacerbated by a viral infection. Most sickness symptoms in dogs are moderate and self-limiting, meaning they go away on their own within 7 to 10 days. Some viral infections that cause canine respiratory disorders include the canine influenza virus, canine parainfluenza virus, canine distemper virus, canine herpesvirus, canine respiratory coronavirus, and canine adenovirus type 2. Recently, canine acute respiratory illnesses brought on by group I and II coronaviruses and the influenza A virus have been reported.
Figure 1. Schematic diagram of canine distemper virus (CDV) genome (da Costa, et al., 2021).
Canine Respiratory Virus VN Kit Development
The virus neutralization assay (VN) is used to treat canine respiratory disorders caused by several viral pathogens. It is a diagnostic test that inhibits the viral attachment to the cell in response to infection. Virus Neutralization (VN) assays have been frequently utilized to discover canine respiratory disease-specific antibodies. Using fibroblast as targeted cells, the virus neutralization assay inhibits the viral attachment to the cell and reverse transcription and integration. To find group-specific antibodies against respiratory disorders in dogs, the VN test has been widely used. The virus neutralization method has the advantage of being able to accurately evaluate serum samples that have been highly hemolyzed and cytotoxic. Various antibodies have been found using VN technologies such as the plaque reduction neutralization test and the serum virus neutralization assay (SVM).
BioVenic is a supplier of biological reagents and kits, we devoted a lot of time to developing diagnostic tools and kits for animal diseases. Our assays are developed and processed with higher standards and the results are delivered on time without compromising quality. We have a team with significant expertise in veterinary diagnostic, and we can offer a variety of specialized viral neutralization (VN) kits for the detection of canine respiratory virus. If you have any needs, please feel free to contact us.
Canine Respiratory Virus VN Kits We Can Provide
| Canine parainfluenza virus VN kit | Canine distemper virus VN kit | Canine herpesvirus VN kit |
| Canine respiratory coronavirus VN kit | Other VN kits you need |
Workflow of Canine Respiratory Virus VN Kit Development
| Workflow | Details |
| Virus titration | Stock virus was aliquoted in many tubes, diluted to its working concentration, and stored at a specified temperature. |
| Dilution | The stock virus was diluted in a virus diluent containing trypsin, and quadruplicate titrations were carried out. |
| Incubation | Incubation was carried out using a finite supply of carbon dioxide at a defined temperature and duration. |
| Cell-fixation | The media was taken out of the viral titration. |
| Micro-neutralization assay | Before being used in the test, all serum was treated with a receptor-destroying enzyme and kept at a certain temperature range for a set amount of time. |
Delivery
- A series of VN kits for canine respiratory viruses
- Report on product inspection quality
- Other experimental data you need
Our Advantages
- High specificity
- Viral pathgens or their toxins can be detected
- High sensitivity
- Detect the level of serological cross reactivity
- Reasonable price
References
- da Costa, Vivaldo Gomes, et al., "First complete genome sequence and molecular characterization of Canine morbillivirus isolated in Central Brazil." Scientific Reports 11.1 (2021): 1-10.
- Martella, Vito, Gabrielle Elia, and Canio Buonavoglia. "Canine distemper virus." Veterinary Clinics of North America: Small Animal Practice 38.4 (2008): 787-797.
