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Infectious laryngotracheitis virus (ILTV), first reported in the 1920s, is a DNA virus that is a member of the Herpesviridae family in the subfamily Alphaherpesvirinae. The genome of ILTV is a linear double-stranded DNA approximately 150 kb long that encodes a variety of proteins, including: L, M, H, B, C, K, G, J, D, I, E and others. ILTV can cause a common respiratory disease in chickens. It can cause a series of symptoms, such as breathing difficulties, reduced egg production and high mortality, leading to significant production losses and a significant economic burden on the poultry industry. Therefore, the rapid diagnosis of ILT is important for the timely implementation of control measures to reduce economic losses in poultry farming.
Figure 1. Reactivity of monoclonal antibodies against ILTV glycoproteins gC (A, C) and gJ (B, D, E) (Fuchs, et al. 2007).
Infectious Laryngotracheitis Virus ELISA Kit Development
Currently, the main methods used for ILT diagnosis are virological assays, serological assays and molecular biology assays. ELISA is a simple and effective method for rapid identification of ILTV infection in poultry, allowing for batch emergency screening and can meet high throughput quarantine requirements. ELISA kits using whole virus as antigen are inefficient in detecting viral seroconversion, but ELISA kits for individual ILTV surface proteins allows rapid detection of seroconversion. Therefore, it is necessary to develop an ELISA kit for the rapid diagnosis of infectious laryngotracheitis using a single ILTV protein as an antigen.
BioVenic is a provider of biology reagents & kits, which has been committed to the development of animal virus diagnostic reagents for many years. We have a professional team with extensive experience in protein expression and purification and antibody preparation. We can prepare specific antibodies against the antigens of infectious laryngotracheitis virus and can provide you with a series of customized ELISA kits for the detection of infectious laryngotracheitis virus according to your needs. If you have any needs, please feel free to contact us. We will provide you with high-quality products for animal diagnostics.
The ELISA Kits We Can Develop
| Infectious laryngotracheitis virus B-ELISA kit | Infectious laryngotracheitis virus C-ELISA kit |
| Infectious laryngotracheitis virus D-ELISA kit | Infectious laryngotracheitis virus E-ELISA kit |
| Infectious laryngotracheitis virus J-ELISA kit |
Workflow of ELISA Kit Development
| Workflow | Details |
|---|---|
| Submit ELISA kit development requests | Determine the ELISA development protocol and estimate the cost and cycle based on the assay targets and experimental requirements. |
| Antigen preparation | The B,C,D,E and J antigens were comprehensively analyzed and evaluated to determine the expression intervals of the antigens, and then recombinant proteins with a purity of not less than 90% were prepared for immunization. |
| Antibody preparation | Preparation of antibodies specific for antigens expressed by gB, gC, gD, gE and gJ genes. |
| ELISA kit development | Antibody label, antibody paired screening, method development and optimization, kit production. |
| ELISA kit delivery | We will provide you with customized ELISA kits and complete product quality inspection report. |
Delivery
- A series of ELISA kits for detection infectious laryngotracheitis virus
- Product quality inspection report (COA)
- Other experimental data you need
Our Advantages
- Highly specific for the detection of antibodies against ILTV
- Excellent reproducibility among batches over time
- Professional antibody platform to support ELISA kit development
- Reasonable price and short turnaround time
References
- Kanabagatte Basavarajappa, Mallikarjuna, et al. "Glycoprotein-based enzyme-linked immunosorbent assays for serodiagnosis of infectious laryngotracheitis." Journal of Clinical Microbiology 53.5 (2015): 1727-1730.
- Fuchs, Walter, et al. "Molecular biology of avian infectious laryngotracheitis virus." Veterinary Research 38.2 (2007): 261-279.
