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Equine respiratory diseases are common disorder in horses. Among them, equine herpesvirus infection, equine influenza, and equine viral arteritis are the most notable. Equine herpesvirus type 2 (EHV-2), equine rhinitis virus and reovirus are ubiquitous viral respiratory pathogens and their infections result in minimal clinical disease. However, secondary bacterial infection may cause mucopurulent nasal discharge, depression, persistent fever, abnormal lung sounds, hyperfibrinogenemia, and leukocytosis. It can also result in mucosal bacterial infections (rhinitis and tracheitis) or produce more serious invasive diseases such as pneumonia and pleuropneumonia. Streptococcus equi zooepidemicus is the most common pathogen of the equine lung. Actinobacillus equuli, Bordetella bronchiseptica, Escherichia coli, Pasteurella spp, and Pseudomonas aeruginosa are also being frequently isolated. Outbreaks of equine respiratory disease bring significant losses to the farms every year.
Figure 1. The replication cycle of EHV-1 and EHV-4 in horses (Ma et al., 2013).
Equine Respiratory Pathogens AGID Kit Development
To effectively control the spread of equine respiratory pathogens and reduce economic losses, it is critical to develop a rapid, simple and economical method for the detection of equine respiratory pathogens. In recent years, serological methods, and molecular biological methods have been used to diagnose equine respiratory pathogens in the laboratory. Compared with molecular biological methods (e.g., PCR), agar gel immunodiffusion is a simple and convenient technique. Researchers have developed several agar gel immunodiffusion methods for the detection of equine respiratory pathogens, including radial immunodiffusion enzyme assay, single immunodiffusion assay, double immunodiffusion assay, etc.
For many years, BioVenic has been dedicated to the development of animal diseases diagnostic reagents and kits. Our R&D team is working hard at developing and optimizing agar gel immunodiffusion kits and we have a competent team with considerable experience in veterinary diagnostic and reagent development. According to your specifications, we can provide a variety of customized agar gel immunodiffusion kits for the identification of equine respiratory pathogens. If you have any needs, please feel free to contact us. We will provide you with high-quality products and services.
Equine Respiratory Pathogens AGID Kits We Can Develop
| Bordetella bronchiseptica radial immunodiffusion enzyme assay kit | Bordetella bronchiseptica single immunodiffusion assay kit | Bordetella bronchiseptica double immunodiffusion assay kit |
| Streptococcus equi zooepidemicus radial immunodiffusion enzyme assay kit | Streptococcus equi zooepidemicus single immunodiffusion assay kit | Streptococcus equi zooepidemicus double immunodiffusion assay kit |
| Equine influenza virus radial immunodiffusion enzyme assay kit | Equine influenza virus single immunodiffusion assay kit | Equine influenza virus double immunodiffusion assay kit |
| Equine herpesviruses radial immunodiffusion enzyme assay kit | Equine herpesviruses single immunodiffusion assay kit | Other AGID Kits you need |
Workflow of Equine Respiratory Pathogens AGID Kit Development
| Workflow | Details |
| Pathogen transport medium | Equine respiratory pathogens were transported by using medium. |
| Solution preparation | Tris-buffered tryptose broth was used in a limited concentration. |
| Sample preparation for testing | Sample was prepared for testing with other pathogens. |
| Swab testing | Swab test was performed for tracheal tissues at a specific temperature. |
| Pathogen isolation | Sample prepared for pathogen isolation was inoculated. |
| AGID testing | Antigen was prepared for agar gel immunodiffusion testing. |
Delivery
- A series of AGID kits for equine respiratory pathogens
- Product quality inspection report
- Other experimental data you need
Our Advantages
- High reproducibility between tests
- Excellent diagnostic relative sensitivity and specificity
- Reasonable price and short turnaround time
- Combine AGID with other detection methods to avoid false positives
References
- Ma, Guanggang, Walid Azab, and Nikolaus Osterrieder. "Equine herpesviruses type 1 (EHV-1) and 4 (EHV-4)—masters of co-evolution and a constant threat to equids and beyond." Veterinary Microbiology 167.1-2 (2013): 123-134.
- Allen, George P. "Risk factors for development of neurologic disease after experimental exposure to equine herpesvirus-1 in horses." American Journal of Veterinary Research 69.12 (2008): 1595-1600.
- Allen, G. P., et al. "Prevalence of latent, neuropathogenic equine herpesvirus‐1 in the Thoroughbred broodmare population of central Kentucky." Equine Veterinary Journal 40.2 (2008): 105-110.
