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Corynebacterium pseudotuberculosis is a Gram-positive, facultative anaerobic, pleomorphic intracellular microorganism. It causes caseous lymphadenitis (CLA) in sheep and goats, which is characterized by chronic suppurative inflammation. This disease can stun sheep growth, reduce reproductive rates, and affects meat quality and milk production. CLA occurs around the world and has a high prevalence. Once the disease occurs in sheep, it is difficult to eradicate and seriously endangers sheep production on a large scale and is one of the major causes of economic losses in sheep farming.
Figure 1. Distribution pattern of common swelling caused by caseous lymphadenitis (CLA) (Osman, et al.2018).
Caseous Lymphadenitis ELISA Kit Development
Currently, the diagnosis of CLA in small ruminants is based on characteristic clinical symptoms and microbial identification of Corynebacterium pseudotuberculosis in abscess sites. However, the diagnosis of asymptomatic animals is crucial for effective control of CLA, since infected animals without obvious symptoms are a source of infection for healthy animals. Serological assays, particularly enzyme-linked immunosorbent assays (ELISA), can be effective in detecting cases of CLA (asymptomatic or non-symptomatic). Researchers have developed a series of ELISA kits that are highly sensitive, reproducible and diagnostically efficient for different antigens.
BioVenic is a provider of biology reagents & kits, which has been committed to the development of animal pathogen diagnostic reagents for many years. We have a professional team with extensive experience in protein expression and purification and antibody preparation. We can prepare antibodies specific for different antigens of Corynebacterium pseudotuberculosis, and we can provide you with a range of customized ELISA kits for Corynebacterium pseudotuberculosis detection according to your needs. If you have any needs, please feel free to contact us. We will provide you with high-quality products for diagnosis of animal diseases.
The ELISA Kits We Can Develop
| Caseous lymphadenitis DTxR ELISA kit | Caseous lymphadenitis Trx ELISA kit | Caseous lymphadenitis TrxR ELISA kit |
| Caseous lymphadenitis LexA ELISA kit | Caseous lymphadenitis SodC ELISA kit | Caseous lymphadenitis SpaC ELISA kit |
| Caseous lymphadenitis NanH ELISA kit | Caseous lymphadenitis PknG ELISA kit | Other caseous lymphadenitisELISA kit |
Workflow of ELISA Kit Development
| Workflow | Details |
| Submit ELISA kit development requests | Determine the ELISA development protocol and estimate the cost and cycle based on the assay targets and experimental requirements. |
| Antigen preparation | Bacteria strains and culture, DNA extraction, polymerase chain reaction and gene cloning, expression and purification antigens of Corynebacterium pseudotuberculosis. |
| Antibody preparation | Preparation of antibodies specific for antigens of Corynebacterium pseudotuberculosis, analysis the specificity and epitope of antibodies. |
| ELISA kit development | Optimizing the best concentration of antigens of Corynebacterium pseudotuberculosis, evaluation of sensitivity, specificity and concordance of ELISA kit. |
| ELISAkit delivery | We will provide you with customized ELISA kits and product quality inspection report. |
Delivery
A series of ELISA kits for detection Corynebacterium pseudotuberculosis
Product quality inspection report (COA)
Other experimental data you need
Our Advantages
- Highly specificity, low background
- Each kit is rigorously validated and tested
- Quantitative measurement of Corynebacterium pseudotuberculosis in multiple samples
- Enables differentiation of weak from strong positive samples
- Reasonable price and short turnaround time
References
- de Farias, Antonio Pedro Fróes, et al. "rSodC is a potential antigen to diagnose Corynebacterium pseudotuberculosis by enzyme-linked immunoassay." AMB Express 10.1 (2020): 1-8.
- Rebouças, Miriam F., et al. "Development of an indirect ELISA to detect Corynebacterium pseudotuberculosis specific antibodies in sheep employing T1 strain culture supernatant as antigen." Pesquisa Veterinária Brasileira 33.11 (2013): 1296-1302.
- Osman, Abdinasir Yusuf. "The epidemiology and pathophysiology of caseous lymphadenitis: A review." Journal of Veterinary Medicine and Research (2018).
