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Western equine encephalitis virus (WEEV) was first isolated from the brains of sick horses in California in 1930. To date, the major equine encephalitis viruses include Venezuelan equine encephalitis virus (VEEV), western equine encephalitis virus (WEEV) and eastern equine encephalitis virus (EEEV). These three viruses are transmitted by bloodthirsty mosquitoes within bird or rodent populations and can cause equine encephalitis. Equine encephalitis is endemic in North, Central and South America. According to statistics, the fatality rate of EEEV is about 30-70%, that of WEEV is about 3%, and that of VEEV is about 1%.
Figure 1. Transmission cycles of Venezuelan equine encephalitis viruses (VEEV), Western equine encephalitis viruses (EEEV), and Eastern equine encephalitis viruses (WEEV) (Ronca, et al. 2016).
Equine Encephalitis Virus PCR Kit Development
Equine encephalitis virus can invade the central nervous system, causing serious complications and death. The spread of equine encephalitis virus has caused huge economic losses to the animal husbandry industry, as well as a significant impact on human health. Therefore, the development of rapid and accurate methods to detect equine encephalitis virus is crucial for the prevention and treatment of equine encephalitis. A variety of equine encephalitis virus detection methods have been developed, including IFA, ELISA, virus isolation and PCR. Compared with traditional methods, PCR has the advantages of high speed and high sensitivity, and has been widely used in the detection of equine encephalitis virus. Commonly used PCR detection methods include RT-PCR, RT-qPCR, RT-nested PCR, duplex real time RT-PCR, etc.
BioVenic is a provider of biology reagents and kits, which has been committed to the development of animal pathogens diagnostic reagents for many years. We have a professional team with extensive experience in veterinary diagnostic and reagent development, and our R&D team is working hard to develop and optimize PCR kits. We can provide you with a series of customized PCR kits for the detection of equine encephalitis virus according to your needs. If you have any needs, please feel free to contact us. We will provide you with high-quality products and services.
Equine Encephalitis Virus PCR Kits We Can Develop
| Equine encephalitis virus RT-PCR kit | Equine encephalitis virus RT-qPCR kit | Equine encephalitis virus RT-nested PCR kit |
| Equine encephalitis virus real-time RT-PCR kit | Equine encephalitis TaqMan one-step RT- PCR kit | Equine encephalitis virus duplex real-time RT-PCR kit |
| Other equine encephalitis virus PCR kits |
Workflow of Equine Encephalitis Virus PCR Kit Development
| Workflow | Details |
| Specimen preparation | Specimens can be collected from horses that were infected by equine encephalitis virus. |
| Primer and probes design | Available sequences of equine encephalitis virus can be retrieved from GenBank and specific primer/probe sets were designed. |
| Nucleic acid extraction | Extract RNA from cell cultures, and RNA concentration, purity and integrity were measured. |
| Establishment of PCR conditions | Establishment of thermocycling conditions to conduct amplification and detection through PCR. |
| Reproducibility evaluation of the PCR assay | The reproducibility of the PCR kit was assessed by determining the intra-assay and inter-assay coefficients of variation (CV). |
| Assessment of sensitivity and specificity | The specificity of PCR kit was assessed by detection of RNA from equine encephalitis virus. The sensitivity of PCR kit was assessed by determine the limit of detection (LoD). |
Delivery
- A series of PCR kits for equine encephalitis virus
- Product quality inspection report
- Other experimental data you need
Our Advantages
- High reproducibility between tests
- High sensitivity to detect very small amounts of equine encephalitis virus in your samples
- Excellent specificity for the detection of equine encephalitis virus
- Reasonable price and short turnaround time
References
- Vina-Rodriguez, Ariel, et al. "A quantitative real-time RT-PCR assay for the detection of Venezuelan equine encephalitis virus utilizing a universal alphavirus control RNA." BioMed Research International 2016 (2016).
- Kang, Xiaoping, et al. "A duplex real-time reverse transcriptase polymerase chain reaction assay for detecting western equine and eastern equine encephalitis viruses." Virology Journal 7.1 (2010): 1-5.
- Ronca, Shannon E., Kelly T. Dineley, and Slobodan Paessler. "Neurological sequelae resulting from encephalitic alphavirus infection." Frontiers in Microbiology 7 (2016): 959.
